| 引用本文: | 谢彩玲,陈念,潘柯伊,王乾蕾.25份燕窝样品的线粒体细胞色素氧化酶I基因序列分析[J].中国现代应用药学,2022,39(3):334-338. |
| XIE Cailing,CHEN Nian,PAN Keyi,WANG Qianlei.Analysis of Mitochondria Cytochrome Oxidase Subunit I Gene of 25 Samples of Bird's Nests[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(3):334-338. |
|
| 摘要: |
| 目的 对多种燕窝进行DNA条形码鉴别，为确定燕窝的基原提供分子依据。方法 对25份燕窝样品的细胞色素氧化酶I（mitochondria cytochrome oxidase subunit I gene，COI）条形码序列进行PCR扩增和测序，分析燕窝正品来源的种内变异，与混伪品的种间变异，以及序列相似性和进化树研究，用Taxon DNA软件评估"Barcoding Gap"。结果 金丝燕种内COI序列变异小，种间存在较多的变异位点，种间的遗传距离显著大于种内的遗传距离。通过构建的系统聚类树图可以看出，燕窝不同来源个体均聚在一起，能够与其混伪品区分开。结论 基于COI序列的DNA条形码技术可以用于鉴定燕窝的正品来源及其混伪品。 |
| 关键词: 燕窝 DNA条形码 线粒体细胞色素氧化酶I基因 鉴定 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.03.008 |
| 分类号:R966 |
| 基金项目:广东省教育厅基础研究重大项目及应用研究重大项目（自然科学）（2017GKZDXM014）；中山市社会公益科技研究项目（2016B2125） |
|
| Analysis of Mitochondria Cytochrome Oxidase Subunit I Gene of 25 Samples of Bird's Nests |
|
XIE Cailing1,2, CHEN Nian1, PAN Keyi3, WANG Qianlei3
|
|
1.Department of Health Industry, Zhongshan Torch Polytechnic, Zhongshan 528436, China;2.Zhongshan health products center, National Engineering Research Center for modernization of traditional Chinese Medicine, Zhongshan 528436, China;3.Zhongshan Institute for Food and Drug Control, Zhongshan 528400, China
|
| Abstract: |
| OBJECTIVE To identify DNA barcodes of bird's nest to provide molecular basis for the substrate of bird's nest. METHODS Mitochondria cytochrome oxidase subunit I gene(COI) fraction from 25 bird's nest samples was amplified by PCR method and sequenced. Then the intraspecific variation among the bird's nest samples and interspecific variation between bird's nest samples and fakes were analysed. Furthermore, the Barcoding Gap was evaluated by using Taxon DNA software. RESULTS the intraspecific variation among swiftlet was small. While many variation loci were found in different swiftlet species, and the genetic distance between species was significantly greater than the intraspecific genetic distance. Phylogenetic tree was constructed, in which the bird's nest samples from different sources were clustered together, while the fakes were distinguished from the bird's nest samples. CONCLUSION DNA barcode technology based on COI sequence can be used to distinguish the original source of bird's nest and its confounding products. |
| Key words: bird's nest DNA barcode COI identification |
