| 引用本文: | 雷蓉,刘亚茹,赵振霞,刘永利.基于UPLC-MS /MS 法的参麦颗粒中麦冬掺伪检查方法研究[J].中国现代应用药学,2025,42(8):134-139. |
| leirong,liuyaru,zhaozhenxia,liuyongli.Study on the adulteration of Ophiopogon Radix in Shenmai Granules based on UPLC-MS/MSLEI Rong,LIU Ya-ru,Zhao Zhen-xia,LIU Yong-li*(Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of quality evaluation and standard of traditional Chinese Medicine,Shijiazhuang050227,China)[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(8):134-139. |
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| 摘要: |
| 目的 建立超高效液相色谱-三重四极杆质谱(UPLC-MS/MS)的参麦颗粒中麦冬掺伪检查方法,考察生产企业是否存在山麦冬掺伪麦冬投料情况。方法 以山麦冬中特征成分短葶山麦冬皂苷C、山麦冬皂苷B为检测指标,分析采用Shimadzu Shim-pack gist C18(2.1×100mm,2μm) 色谱柱,以0.1%甲酸水溶液-乙腈为流动相等度洗脱,体积流量为 0.3 mL.min-1,柱温 40 ℃。电喷雾正离子源(ESI+)扫描,多反应监测(MRM)模式。结果 短葶山麦冬皂苷C、山麦冬皂苷B分别在3.290～315.8n ng.mL-1、 2.390～305.9 ng.mL-1呈良好的线性关系,相关系数均大于 0.9996,加样回收率为105.3%、100.6%,精密度、重复性和稳定性的RSD均小于3.0%。85批参麦颗粒样品中有8批检出山麦冬皂苷B,检出率为9.4%。结论 建立的方法简便快速、准确性好、专属性强,可用于筛查参麦颗粒中麦冬的掺伪情况。 |
| 关键词: 参麦颗粒 麦冬 山麦冬 山麦冬皂苷B 短葶山麦冬皂苷C |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目: |
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| Study on the adulteration of Ophiopogon Radix in Shenmai Granules based on UPLC-MS/MSLEI Rong,LIU Ya-ru,Zhao Zhen-xia,LIU Yong-li*(Hebei Institute for Drug and Medical Device Control, Hebei Key Laboratory of quality evaluation and standard of traditional Chinese Medicine,Shijiazhuang050227,China) |
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leirong, liuyaru, zhaozhenxia, liuyongli
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Hebei Institute for Drug and Medical Device Control
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| Abstract: |
| OBJECTIVE To establish a method for the adulteration of Ophiopogonis Radix in Shenmai Granules to investigate whether there is a phenomenon of using Liriope Radix to replace Ophiopogon Radix in the manufacture enterprises. METHODS The characteristic components liriope muscari baily saponins C and liriopesides B were used as the detection index of Liriope Radix. The sample extract was cleaned up by a C18 solid-phase extraction cartridge combined with UPLC-MS/MS. Separation was performed on a Waters Acquity BEH C18 (2.1 mm×100 mm, 1.7 μm) with a gradient elution of acetonitrile and 0.1% formic acid at the flow rate was set at 0.3 mL·min-1 and a column temperature of 40 ℃. The detection mode was Multi reaction monitoring (MRM) in electrospray positive ion source (ESI+). RESULTS Liriope muscari baily saponins C and liriopesides B showed good linearity in the range of 3.290～315.8n ng·mL-1 and 2.390～305.9 ng·mL-1, with the correlation coefficients greater than 0.9996, and the recoveries of 105.3% and 100.6%, and the RSDs of precision, reproducibility, and stability were less than 3.0%. 85 batches of Shenmai Granules contained 8 batches with the detection rate of 9.4%.
CONCLUSION The established method is simple, rapid, accurate and exclusive, and can be used to screen the adulteration of Ophiopogon japonicus in Shenmai granules |
| Key words: Shenmai Granules Ophiopogon japonicus Liriope Liriopesides B Liriope muscari baily saponins C |
