银基碳纳米颗粒的制备与抗菌性能

李健; 赵向东; 田景民; 马广娜; 张慧文; 吕微; 武世奎

引用本文:	李健,赵向东,田景民,马广娜,张慧文,吕微,武世奎.银基碳纳米颗粒的制备与抗菌性能[J].中国现代应用药学,2025,42(8):126-133.
	lijian,zhaoxiangdong,tianjingmin,maguangna,zhanghuiwen,lvwei,wushikui.Preparation nd Antibacterial Properties of Silver-based Carbon Nanoparticles[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(8):126-133.

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银基碳纳米颗粒的制备与抗菌性能
李健¹, 赵向东², 田景民¹, 马广娜¹, 张慧文¹, 吕微¹, 武世奎¹
1.内蒙古医科大学药学院;2.内蒙古医科大学附属医院

摘要:

摘要：目的：探索一种制备简单且具有良好抗菌效果的银基碳纳米颗粒 (Silver-based carbon nanoparticles, Sbc-Nps) 。方法：首先以硝酸银、4-二甲氨基吡啶为基础原料制得 4-二甲氨基吡啶-银配合物纳米颗粒 (4-Dimethylaminopyridine-silver, Dap-Ag) 作为煅烧前驱体，该过程使用聚乙烯吡咯烷酮K30调节Dap-Ag的粒径；继而通过差示扫描量热仪判断煅烧过程的最佳温度变化条件，将Dap-Ag在氮气氛围内煅烧制得Sbc-Nps。其次分别用耐甲氧西林金黄色葡萄球菌 (Methicillin-resistant Staphylococcus aureus, MRSA) 和表皮葡萄球菌 (Staphylococcus epidermidis, SE) 测试Sbc-Nps的抗菌效果。最后通过HaCaT细胞毒性实验测试Sbc-Nps的安全性。结果：得到粒径100-200 nm且银元素高度富集的Sbc-Nps，其在0.125 mg·ml-1和0.25 mg·ml-1的浓度时可分别对MRSA和SE达到较好的抑菌效果，且煅烧方法使Sbc-Nps的细胞毒性显著降低。结论：Sbc-Nps不仅制备简单、粒径较小、银元素富集，而且具有良好的抗菌性能和生物相容性。

关键词: 吡啶二价银配合物、银基碳纳米颗粒、低真空度-氮气氛围煅烧、抗菌性能、生物相容性

DOI：

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基金项目:内蒙古自治区高等学校“创新团队发展计划”：中(蒙)药微纳米结构与药效NMGIRT2421；国家自然科学：蒙药孟根乌苏中硫化汞纳米颗粒的经皮靶向给药及其减毒机制研究,项目编号：82360824；内蒙古自治区科技计划项目：蒙药经典名方孟根乌苏-18 味丸的二次开发,项目编号：2023YFHH0018；中央引导地方科技发展资金项目：活性氧响应的硫化汞纳米颗粒的构筑及其对银屑病治疗的抗原递呈机制研究. 项目编号：2022ZY0176。

Preparation nd Antibacterial Properties of Silver-based Carbon Nanoparticles

lijian¹, zhaoxiangdong², tianjingmin¹, maguangna¹, zhanghuiwen¹, lvwei¹, wushikui¹

1.College of pharmacy, Inner Mongolia Medical University;2.Hospital of Inner Mongolia Medical University

Abstract:

Abstracts: OBJECTIVE: To explore a silver-based carbon nanoparticle (Sbc-Nps) with easy preparation and good antibacterial activity. METHODS: 4-Dimethylaminopyridine-silver complex nanoparticles (Dap-Ag) were first prepared from silver nitrate and 4-dimethylaminopyridine as basic raw materials as a precursor for calcination, and the process was used to regulate the particle size of Dap-Ag using polyvinylpyrrolidone K30; Following the optimum temperature change conditions for the calcination process determined by differential scanning calorimetry, Sbc-Nps was prepared by calcining Dap-Ag in a nitrogen atmosphere. The antimicrobial activity of Sbc-Nps was then tested against Methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (SE). Finally, the safety of Sbc-Nps was assessed using the HaCaT cytotoxicity assay. RESULTS: Sbc-Nps with particle sizes of 100-200 nm and highly enriched in silver elements were obtained, which could achieve better inhibitory effects against MRSA and SE at concentrations of 0.125 mg·ml-1 and 0.25 mg·ml-1, respectively, and the cytotoxicity of Sbc-Nps was significantly reduced by the calcination method. CONCLUSION: Sbc-Nps is not only easy to prepare, has a small particle size and is enriched with silver, but also has excellent antimicrobial properties and biocompatibility.

Key words: pyridine divalent silver complexes, silver-based carbon nanoparticles, low vacuum nitrogen atmosphere calcination, antibacterial properties