基于UPLC-QQQ-MS法和PLS-DA分析的2种寄主鲜品肉苁蓉苯乙醇苷成分差异研究

吕霞; 郭晔红; 高培文; 张文瑶; 王怡维; 贾存勤

引用本文:	吕霞,郭晔红,高培文,张文瑶,王怡维,贾存勤.基于UPLC-QQQ-MS法和PLS-DA分析的2种寄主鲜品肉苁蓉苯乙醇苷成分差异研究[J].中国现代应用药学,2025,42(9):51-58.
	LV Xia,GUO Yehong,GAO Peiwen,ZHANG Wenyao,WANG Yiwei,JIA Cunqin.Study on the difference of phenylethanol glycosides in 2 hosts table Cistanches Herba based on UPLC-QQQ-MS method and PLS-DA analysis[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(9):51-58.

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基于UPLC-QQQ-MS法和PLS-DA分析的2种寄主鲜品肉苁蓉苯乙醇苷成分差异研究
吕霞¹, 郭晔红¹, 高培文¹, 张文瑶¹, 王怡维¹, 贾存勤²
1.甘肃农业大学农学院，干旱生境作物学国家重点实验室;2.甘肃汇勤生物科技有限公司

摘要:

目的:比较评价鲜品四翅滨藜-肉苁蓉与梭梭-肉苁蓉汁和渣中苯乙醇苷成分含量的差异。方法:通过超高效液相色谱-三重四极杆串联质谱联用法(UPLC-QQQ-MS),对2种寄主鲜品肉苁蓉汁和渣中8种苯乙醇苷成分含量进行比较,并结合偏最小二乘判别分析(partial least-squares discriminant-analysis, PLS-DA),鉴定并评价2种寄主鲜品肉苁蓉的苯乙醇苷成分差异。结果:四翅滨藜-肉苁蓉汁和渣中的苯乙醇苷含量均高于梭梭-肉苁蓉；经过杀酶5 min的四翅滨藜-肉苁蓉和梭梭-肉苁蓉渣,再经80℃干燥后的肉苁蓉苯乙醇苷含量较未杀酶直接干燥的肉苁蓉分别高出131.51%和93.32%。结论:以肉苁蓉中的主要成分苯乙醇苷为评判指标,可快速、高效地评估鲜品四翅滨藜-肉苁蓉与梭梭-肉苁蓉汁与渣中活性成分的差异。且鲜品四翅滨藜-肉苁蓉渣的苯乙醇苷含量更高,杀酶5min可有效保留鲜品肉苁蓉苯乙醇苷的含量。

关键词: UPLC-QQQ-MS 四翅滨藜梭梭苯乙醇苷鲜品肉苁蓉

DOI：

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基金项目:国家自然科学基金(31860349)；甘肃省科技计划(18YFlNA072, 21CX6NH279)

Study on the difference of phenylethanol glycosides in 2 hosts table Cistanches Herba based on UPLC-QQQ-MS method and PLS-DA analysis

LV Xia¹, GUO Yehong¹, GAO Peiwen¹, ZHANG Wenyao¹, WANG Yiwei¹, JIA Cunqin²

1.Agronomy College, Gansu Agricuitural university，National Key Laboratory of Aridland Crop Science;2.Gansu Huiqin Biological Technology Co., Ltd.

Abstract:

Objective: To comparatively evaluate the differences in the content of phenylethanol glycosides in the juice and residue of table Cistanches Herba with the host of Atriplex canescens and with the host of Haloxylon ammodendron. Methods: Comparative evaluation of the differences in the content of 8 phenylethanol glycoside components in the juice and residue of the two hosts fresh Cistanches Herba by ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QQQ-MS) and combining with partial least squares discriminant-analysis (PLS-DA). Results: The contents of phenylethanol glycosides in the juice and residue of fresh Cistanches Herba with the host of A. canescens were higher than those in Cistanches Herba with the host of H. ammodendron. The phenylethanol glycoside content of A. canescens-Cistanches Herba and H. ammodendron-Cistanches Herba residues, which were enzyme-killed for 5 min and then dried at 80°C, was 131.51% and 93.32% higher than that of the direct-dried without enzyme-killing, respectively. Conclusion: Using phenylethanol glycosides, the main components in Cistanches Herba as a judgement index, the differences in active ingredients in the juice and residue of fresh A. canescens-Cistanches Herba and H. ammodendron -Cistanches Herba can be assessed quickly and efficiently. And the content of phenylethanol glycosides is higher in fresh Cistanches Herba residues, and killing the enzyme for 5 min could effectively retain the content of phenylethanol glycosides in fresh Cistanche Herba.

Key words: UPLC-QQQ-MS Atriplex canescens Haloxylon ammodendron phenylethanol glycosides table Cistanches Herba