| 引用本文: | 邓其奕,卜佳伟,孙玉莹,程芷芸,张自鹏,万义超,侯梦飞.树状大分子作为IR820纳米载体在肿瘤光热治疗中的应用[J].中国现代应用药学,2025,42(2):37-43. |
| dengqiyi,bujiawei,sunyuying,chengzhiyun,zhangzipeng,wanyichao,houmengfei.Utilizing Dendrimers as Nanocarriers for IR820 in Photothermal Therapy of Tumors[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(2):37-43. |
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| 树状大分子作为IR820纳米载体在肿瘤光热治疗中的应用 |
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邓其奕,卜佳伟,孙玉莹,程芷芸,张自鹏,万义超,侯梦飞
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1.湖南科技大学化学化工学院理论有机化学与功能分子教育部重点实验室;2.山东大学药学院;3.山东第一医科大学(山东省医学科学院)药学院临床药学系;4.山东第一医科大学(山东省医学科学院)医学科技创新中心;5.山东第一医科大学(山东省医学科学院)放射学院
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| 摘要: |
| 目的 光热疗法是一种新型的抗肿瘤治疗方法。然而,所使用的光敏剂在体内寿命短,在肿瘤部位的积累有限。因此,需要新的药物输送系统来增强光热治疗。本研究利用树状大分子PAMAM作为光敏剂IR820的纳米载体,对肿瘤进行光热治疗。方法 采用共价共轭法制备了负载IR820的纳米颗粒。采用核磁共振氢谱(1H-NMR)对纳米颗粒进行表征。通过监测纳米颗粒在激光照射下的温度变化来评价纳米颗粒的体外光热性能。采用CCK8法评估纳米颗粒对MC38肿瘤细胞的细胞毒性。进一步进行体内实验,评价纳米颗粒在光热治疗中的抗肿瘤作用。结果 分析结果表明,IR820成功加载到纳米颗粒上。纳米颗粒在近红外(NIR)激光照射下,肿瘤细胞温度显著升高,显示出细胞毒性活性。在荷瘤小鼠模型中,纳米颗粒在近红外激光照射下显著抑制肿瘤生长,减小肿瘤体积,但对小鼠体重无显著影响,说明纳米颗粒具有良好的抗肿瘤作用和体内安全性。结论 负载IR820的PAMAM可以作为纳米载体增强抗肿瘤光热治疗的疗效。 |
| 关键词: 第五代聚酰胺胺(PAMAM)树状大分子 新型吲哚菁绿(IR820)光敏剂 光热治疗 肿瘤细胞 |
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| 基金项目:国家自然科学基金项目(82003602, 22277025)；湖南省自然科学基金项目(2023JJ30270)；湖南省教育厅科研基金项目(21B0478)；湖南省科技创新计划项目(2021RC5028)；湖南省大学生创新创业训练项目(S202310534065) |
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| Utilizing Dendrimers as Nanocarriers for IR820 in Photothermal Therapy of Tumors |
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dengqiyi1, bujiawei2, sunyuying3,4, chengzhiyun1, zhangzipeng5,4, wanyichao6, houmengfei7,4
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1.Key Laboratory of Theoretical Organic Chemistry and Functional Molecule, Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology,;2.Department of Pharmacy,Shandong University;3.Department of Clinical Pharmacy, School of Pharmacy, Shandong First Medical University &4.Shandong Academy of Medical Sciences;5.Medical Science and Technology Innovation Center Shandong First Medical University &6.Key Laboratory of Theoretical Organic Chemistry and Functional Molecule, Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology;7.School of Radiology, Shandong First Medical University &
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| Abstract: |
| ABSTRACT: OBJECTIVE Photothermal therapy is a novel anti-tumor treatment method. However, the photosensitizers used have a short lifespan in vivo and limited accumulation at the tumor site. Therefore, new drug delivery systems are required to enhance photothermal therapy. In this study, dendrimers PAMAM was utilized as a nanocarrier for the photosensitizer IR820 in photothermal therapy for tumors. METHODS Nanoparticles loaded with IR820 were prepared using a covalent conjugation method. Characterization was conducted using proton nuclear magnetic resonance (1H-NMR) The in vitro photothermal performance of the nanoparticles was evaluated by monitoring the temperature changes of the nanoparticles under laser irradiation. The cytotoxicity of the nanoparticles on MC38 tumor cells was assessed using the CCK8 assay. Further in vivo experiments were conducted to evaluate the antitumor efficacy of nanoparticle in photothermal therapy. RESULTS The analysis results demonstrate successful loading of IR820 onto nanoparticles. The nanoparticles exhibit a significant increase in tumor cell temperature under near-infrared (NIR) laser irradiation, demonstrating cytotoxic activity. In the tumor-bearing mice model, nanoparticles significantly inhibited tumor growth and reduced tumor volume under NIR laser irradiation, but there was no significant difference in mice body weight, indicating that nanoparticle has a good anti-tumor effect and in vivo safety. CONCLUSION PAMAM loaded with IR820 could serve as a nanocarriers to enhance anti-tumor photothermal therapy. |
| Key words: fifth-generation polyamidoamine (PAMAM) dendrimers new indocyanine green (IR820) photosensitizer photothermal therapy tumor cells |
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