| 引用本文: | 吴迪,徐继玉,王路雅,伍立,李嘉欣,班玛才郎,赵晓辉,张得钧,岳会兰.大果白刺通过PI3K/AKT信号通路改善2型糖尿病小鼠胰岛抵抗[J].中国现代应用药学,2025,42(8):81-93. |
| Wu Di,Xu Jiyu,Wang Luya,Wu Li,lijiaxin,Banma Cailang,Zhao Xiaohui,Zhang Dejun,Yue Huilan.Improvement effect of insulin resistance of Nitraria roborowskii Kom in type 2 diabetic mice via PI3K/AKT signaling pathway[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(8):81-93. |
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| 大果白刺通过PI3K/AKT信号通路改善2型糖尿病小鼠胰岛抵抗 |
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吴迪1, 徐继玉1, 王路雅2, 伍立2, 李嘉欣2, 班玛才郎2, 赵晓辉2, 张得钧1, 岳会兰2
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1.青海大学;2.中国科学院西北高原生物研究所
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| 摘要: |
| Nitraria roborowskii Kom。（NRK）作为Nitraria属的果实，已被广泛用作治疗糖尿病的民间药物。然而，关于NRK的现代低血糖活性和潜在机制的研究仍然非常缺乏。
目的：评估NRK-C在2型糖尿病（T2DM）中的降血糖作用及其可能机制。
方法：通过高脂饮食（HFD）联合6次腹腔注射35 mg/kg链脲佐菌素（STZ）诱导的T2DM小鼠的体重、器官指标、血糖水平、血清生化指标以及HE/PAS组织病理切片。进一步应用Western blot和免疫荧光法确定NRK-C对关键信号蛋白的调控作用。
结果：NRK-C给药7周后空腹血糖水平显著降低。此外，NRK-C还能显著改善糖尿病小鼠的葡萄糖耐量、肝糖原水平和血脂水平（TG、TC、LDL-C和HDL-C），显著降低糖尿病小鼠的胰岛素抵抗（IR），在抗糖尿病作用中发挥重要作用。进一步的机制研究表明，NRK-C干预后磷酸化Akt表达上调，GSK3β表达下调，表明NRK-C可能通过调节PI3K/Akt信号通路发挥潜在的抗糖尿病作用。
结论：所有这些结果都表明NRK-C可能改善T2DM，并具有作为辅助治疗的潜力。 |
| 关键词: 2型糖尿病 Nitraria roborowskii Kom 葡萄糖耐量 胰岛素抵抗 PI3K/AKT信号通路 |
| DOI：10.13748/j.cnki.issn1007-7693.20232480 |
| 分类号:R969.3 |
| 基金项目:本研究由青海省成果转化项目（2021-SF-145）和国家自然科学基金（31900298）资助。 |
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| Improvement effect of insulin resistance of Nitraria roborowskii Kom in type 2 diabetic mice via PI3K/AKT signaling pathway |
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Wu Di1, Xu Jiyu1, Wang Luya2, Wu Li2, lijiaxin2, Banma Cailang2, Zhao Xiaohui2, Zhang Dejun1, Yue Huilan2
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1.Qinghai University;2.Northwest Plateau Biology Institute, Chinese Academy of Sciences
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| Abstract: |
| Ethnopharmacological relevance: Nitraria roborowskii Kom. (NRK), as the fruit of the genus Nitraria, has been widely used as folk medicine to treat diabetes. However, Studies for modern hypoglycemic activity and underlying mechanism of NRK are still very lacking.
Aim: The hypoglycaemic effect of NRK-C and its possible mechanism was evaluated in the type 2 diabetes mellitus (T2DM).
Methods: The body weight, organ indices, blood glucose levels, serum biochemical indexes, as well as HE/PAS histopathological section were all analyzed to assess the hypoglycaemic effect of NRK-C in T2DM mice induced by a high-fat diet (HFD) combined with six intraperitoneal injections of 35 mg/kg of streptozotocin (STZ). The Western blot and immunofluorescence were further applied to determine the regulatory effect of NRK-C on key signaling proteins.
Results: The fasting blood glucose levels were significantly reduced after 7 weeks of administration of NRK-C. In addition, NRK-C could also significantly improve glucose tolerance, hepatic glycogen levels, and lipid levels (TG, TC, LDL-C, and HDL-C), and significantly reduced insulin resistance (IR) of diabetic mice, which played an important role in the antidiabetic effects. Further mechanism research demonstrated that phosphorylated Akt expression was up-regulated and GSK3β expression was down-regulated after NRK-C intervention, indicating that NRK-C might exert a potential antidiabetic effect by modulating the PI3K/Akt signaling pathway.
Conclusion: All these results suggested that NRK-C might improve T2DM and had the potential to be used as an adjunctive therapy. |
| Key words: Type 2 diabetes Nitraria roborowskii Kom Glucose tolerance Insulin resistance PI3K/AKT signaling pathway |
