| 引用本文: | 毛菊华,潘俊杰,陈张金,吴查青,王伟影,程科军.基于UHPLC-MS/MS的食凉茶2种基原植物不同部位中26个成分的含量对比分析[J].中国现代应用药学,2024,41(4):489-495. |
| MAO Juhua,PAN Junjie,CHEN Zhangjin,WU Chaqing,WANG Weiying,CHENG Kejun.Determination and Comparison of 26 Components in Different Parts of Two Base Plants of Shiliang Tea Based on UHPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(4):489-495. |
|
| 摘要: |
| 目的 建立一种超高效液相色谱-串联质谱(UHPLC-MS/MS)法测定和对比食凉茶2种基原植物(柳叶蜡梅Chimonanthus salicifolius S.Y.Hu和浙江蜡梅Chimonanthus zhejiangensis M.C.Liu)不同部位根、茎、叶中26个成分的含量，以筛选不同部位的质量标志物。方法 UHPLC采用Agilent RRHD Eclipse Plus C18(2.1 mm×50 mm，1.8 μm)色谱柱，流动相为甲醇-0.1%甲酸水溶液，梯度洗脱，流速0.3 mL· min?1，柱温35 ℃，进样量0.5 μL；三重四级杆质谱采用电喷雾离子源(ESI)正负离子模式，通过多重反应监测模式对柳叶蜡梅和浙江蜡梅不同部位中26个化学成分进行定量分析。结果 26个成分在各自浓度范围内线性关系良好(r >0.999)，平均加样回收率为88.5%~111.7%，RSD为3.4%~9.8%。食凉茶2种基原植物中各成分的含量无明显差异，聚类分析将叶和根、茎分为两大类，表明两者含量差异较大，其中叶均以黄酮类成分为主，以山柰酚-3-O-芸香糖苷含量最高，达12.902 mg·g?1；茎和根均以香豆素类成分为主，根的生物碱含量相对更高；经正交偏最小二乘法-判别分析，筛选出山柰酚-3-O-芸香糖苷、山蜡梅碱、芦丁、秦皮苷、异秦皮啶-7-O-β-D-葡萄糖苷、东莨菪苷、新绿原酸7个质量差异标志物。结论 本研究阐明了食凉茶2种基原植物不同部位的化学成分差异，为进一步研究柳叶蜡梅和浙江蜡梅药效物质基础奠定了依据，同时为其全资源利用提供了参考。 |
| 关键词: 食凉茶 柳叶蜡梅 浙江蜡梅 不同部位 根 茎 叶 聚类分析 正交偏最小二乘法-判别分析 超高效液相色谱-串联质谱 |
| DOI：10.13748/j.cnki.issn1007-7693.20230992 |
| 分类号:R284.1 |
| 基金项目:丽水市重点研发计划项目(2020ZDYF04)；丽水市公益性技术应用研究项目(2023SJZC053) |
|
| Determination and Comparison of 26 Components in Different Parts of Two Base Plants of Shiliang Tea Based on UHPLC-MS/MS |
|
MAO Juhua1, PAN Junjie2, CHEN Zhangjin1, WU Chaqing1, WANG Weiying1,3, CHENG Kejun2
|
|
1.Lishui Institute for Quality Inspection and Testing, Lishui 323000, China;2.Lishui Institute of Agriculture and Forestry Sciences, Lishui 323000, China;3.Zhejiang Chinese Medical University, Hangzhou 310053, China
|
| Abstract: |
| OBJECTIVE To establish an ultra-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method for determination and comparison of 26 components in different parts of two base plants of Shiliang tea(Chimonanthus salicifolius S.Y.Hu and Chimonanthus zhejiangensis M.C.Liu), and screen quality markers of different parts. METHODS The UHPLC method was performed on an Agilent RRHD Eclipse Plus C18 (2.1 mm×50 mm, 1.8 μm) column with a gradient elution of methanol and 0.1% formic acid in water at a flow rate of 0.3 mL·min?1, the column temperature was 35 ℃, and the injection volume was 0.5 μL; the multiple reaction monitoring mode was employed for the quantification of 26 components with electrospray ionization(ESI) source polarity in negative and positive mode. RESULTS Good linear relationship(r >0.999) were observed in the test ranges for 26 compounds, and the average recovery was 88.5%?111.7% with RSD was 3.4%?9.8%. There was no significant difference between the two base plants of Shiliang tea, and all of these samples were divided into two categories by hierarchical cluster analysis. The main components in leaves was flavonoids, among them, the content of kaempferol 3-O-rutinoside was the highest, reaching 12.902 mg.g?1; the main components in stems and roots was coumarins, and the content of alkaloids in roots was higher, relatively; 7 quality markers of difference were screened by OPLS-DA, which were kaempferol 3-O-rutinoside, chimonanthine, rutin, fraxetin, calycanthoside, scopolin, neochlorogenic acid. CONCLUSION These study elucidates the differences of chemical components in the different parts of two base plants of Shiliang tea, which providing basis for the research of pharmacodynamic substances and references for the comprehensive utilization of Chimonanthus salicifolius S.Y. Hu and Chimonanthus zhejiangensis M.C.Liu resources. |
| Key words: Shiliang tea Chimonanthus salicifolius S.Y. Hu Chimonanthus zhejiangensis M.C.Liu different parts roots stems leaves cluster analysis OPLS-DA UHPLC-MS/MS |
