| 引用本文: | 李莎莎,狄志彪,胡静,李雅娟,毛阿娟,李凡,李芳,张红,王卫锋.基于UHPLC-Q-Exactive Focus-MS/MS的太白茶化学成分研究[J].中国现代应用药学,2024,41(1):71-79. |
| LI Shasha,DI Zhibiao,HU Jing,LI Yajuan,MAO A'juan,LI Fan,LI Fang,ZHANG Hong,WANG Weifeng.Study on Chemical Constituents in Thamnolia Subuliformis (Ehrh.) W.Culb. by UHPLC-Q-Exactive Focus-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(1):71-79. |
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| 基于UHPLC-Q-Exactive Focus-MS/MS的太白茶化学成分研究 |
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李莎莎1, 狄志彪1, 胡静1, 李雅娟2, 毛阿娟1, 李凡1, 李芳1, 张红1, 王卫锋1
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1.陕西省中医药研究院，西安 710003;2.陕西中医药大学，陕西 咸阳 712046
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| 摘要: |
| 目的 应用超高效液相色谱-四级杆-静电场轨道阱高分辨质谱法(UHPLC-Q-Exactive Focus-MS/MS)对太白茶醇提物进行快速定性研究。方法 采用Waters Acquity UPLC BEH C18(2.1 mm×50 mm，1.7 μm)色谱柱，以乙腈(A)-0.1%甲酸水(B)为流动相，梯度洗脱，流速0.3 mL·min–1，柱温30 ℃；质谱条件为电喷雾离子源(ESI)，负离子模式扫描，扫描范围m/z 80~1 200。根据高分辨质谱数据分析，参考文献及对照品确认，对太白茶中的化学成分进行快速、全面的定性分析。结果 从太白茶共鉴定出了39种成分，包括多取代单苯环类化合物9种，缩酚酸类11种，缩酚酸环醚类5种，二苯并呋喃类2种，脂肪酸类10种及其他2种。结论 该方法可快速、全面鉴定太白茶中的化学成分，其中3种成分(苔色酸、松萝酸、黑茶渍素)与对照品比对后准确鉴定，19种成分首次在太白茶中发现，可为太白茶药效物质研究及质量评价提供重要依据。 |
| 关键词: 太白茶 液质联用 化学成分 |
| DOI：10.13748/j.cnki.issn1007-7693.20223535 |
| 分类号:R284.1 |
| 基金项目:陕西省中医药管理局中医药全省性专款专项项目(2021-QYZL-01)；陕西省中医药研究院“苗圃培育计划”项目(2021-16，2021-21)；陕西省中医药管理局传承创新暨“秦药”开发重点科学研究项目(2021-02-GJ-021) |
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| Study on Chemical Constituents in Thamnolia Subuliformis (Ehrh.) W.Culb. by UHPLC-Q-Exactive Focus-MS/MS |
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LI Shasha1, DI Zhibiao1, HU Jing1, LI Yajuan2, MAO A'juan1, LI Fan1, LI Fang1, ZHANG Hong1, WANG Weifeng1
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1.Shaanxi Provincial Academy of Traditional Chinese Medicine, Xi’an 710003, China;2.Shaanxi University of Chinese Medicine, Xianyang 712046, China
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| Abstract: |
| OBJECTIVE To conduct a rapid qualitative study on the chemical constituents of the methanol extract in Thamnolia subuliformis (Ehrh.) W.Culb. by UHPLC-Q-Exactive Focus-MS/MS. METHODS The chromatographic separation was performed on a Waters Acquity UPLC BEH C18(2.1 mm×50 mm, 1.7 μm) column with acetonitrile(A)-0.1% formic acid aqueous solution(B) as mobile phase for gradient elution, the flow rate was 0.3 mL·min–1 and the column temperature was 30 ℃. Mass spectrometry was performed using an electrospray ionization and data was collected in negative ion modes, and the detection range was m/z 80-1 200. The chemical constituents in Thamnolia subuliformis (Ehrh.) W.Culb. were identified rapidly and comprehensively based on the accurate relative molecular and combined with literature data and reference substances. RESULTS A total of 39 chemical constituents were speculatively identified, including 9 mono-substituted phenyl rings, 11 depsides, 5 depsidones, 2 dibenzofuran, 10 lipids, and 2 others. CONCLUSION The chemical constituents in the Thamnolia subuliformis (Ehrh.) W.Culb. can be identified accurately and rapidly by this method. Among them, 3 compounds(β-resorcylic acid, usnic acid, atranorin) are unambiguously identified by comparing with reference standards, 19 compounds are found from Thamnolia subuliformis (Ehrh.) W.Culb. for the first time. This paper can provide the important basis for study on pharmacodynamic material and substitute development of Thamnolia subuliformis (Ehrh.) W.Culb.. |
| Key words: Thamnolia subuliformis (Ehrh.) W.Culb. UHPLC-Q-Exactive Focus-MS/MS chemical constituents |
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