| 引用本文: | 徐杰,甘海宁,黄瑶,梁丽金,纪玉华,张志鹏,孙冬梅.鲜、生地黄配方颗粒特征图谱差异及干预LPS诱导大鼠发热模型的药效比较研究[J].中国现代应用药学,2025,42(8):56-63. |
| xujie,ganhaining,huangyao,lianglijin,jiyuhua,zhangzhipeng,sundongmei.Comparative Study on Characteristic Chromatogram and Effects on LPS Induced Fever Model in Rats of Fresh and Raw Rehmannia glutinosa Formula Granules[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(8):56-63. |
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| 摘要: |
| 摘要：目的 研究鲜地黄配方颗粒与生地黄配方颗粒的特征图谱以及解热作用的差异。方法 采用UPLC法，分别采集3批鲜地黄配方颗粒与9批生地黄配方颗粒环烯醚萜苷类成分和苯乙醇苷类成分的特征图谱，比较二者特征峰数目的差异；选取体温合格的SPF级SD大鼠70只，根据基础体温随机分为7组，分别为正常对照组、模型对照组、布洛芬组、生地黄配方颗粒高剂量组、生地黄配方颗粒低剂量组、鲜地黄配方颗粒高剂量组、鲜地黄配方颗粒低剂量组。除正常对照组外，其余各组按100 μg·kg-1腹腔注射脂多糖（LPS），正常对照组腹腔注射等体积的生理盐水，每隔2 h测1次体温，计算各个监测点体温变化值和体温上升抑制率。结果 与鲜地黄配方颗粒特征图谱相比，生地黄环烯醚萜苷类成分特征图谱缺失峰8，苯乙醇苷类成分特征图谱多检出峰9，此两个特征峰可用于鲜、生地黄配方颗粒的专属性鉴别；LPS造模后2、4、6 h，鲜地黄配方颗粒高剂量组均有较显著的解热作用（P＜0.05），各配方颗粒样品的大鼠体温上升抑制率比较为鲜地黄配方颗粒高剂量＞生地黄配方颗粒高剂量＞鲜地黄配方颗粒低剂量＞生地黄配方颗粒低剂量，鲜地黄配方颗粒对LPS所致大鼠发热模型的解热作用更优。结论 鲜、生地黄配方颗粒特征图谱及解热作用均存在差异，鲜地黄配方颗粒的解热作用更优，研究结果可为鲜地黄配方颗粒的临床推广提供数据支撑。 |
| 关键词: 鲜地黄 生地黄 配方颗粒 环烯醚萜苷 苯乙醇苷 特征图谱 解热作用 |
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| 基金项目:国家工信部2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台（项目编号：2022-230-221） |
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| Comparative Study on Characteristic Chromatogram and Effects on LPS Induced Fever Model in Rats of Fresh and Raw Rehmannia glutinosa Formula Granules |
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xujie1, ganhaining2, huangyao1, lianglijin1, jiyuhua1, zhangzhipeng1, sundongmei1
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1.Guangdong Yifang Pharmaceutical Co., Ltd;2.Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology
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| Abstract: |
| ABSTRACT: OBJECTIVE To study the difference in characteristic chromatograms and antipyretic effects between fresh and raw Rehmannia glutinosa formula granules. METHODS Compared the difference in characteristic chromatograms of iridoid glycosides and phenylethanol glycosides in fresh and raw Rehmannia glutinosa formula granules by using ultra high performance liquid chromatography. Seventy SPF grade SD rats with acceptable body temperature were randomly divided into 7 groups based on their basal body temperature, namely, normal control group, model control group, ibuprofen group, high dose group raw Rehmannia glutinosa formula granules, low dose group of raw Rehmannia glutinosa formula granules, high dose group of fresh Rehmannia glutinosa formula granules, and low dose group fresh Rehmannia glutinosa formula granules. Except for the normal control group, all other groups were injected intraperitoneally with 100 μg·kg-1 lipopolysaccharide (LPS), while the normal control group was injected intraperitoneally with an equal volume of physiological saline. The body temperature was measured every 2 hours, and the temperature change value and the inhibition rate of temperature rise at each monitoring point were calculated. RESULTS Compared with the characteristic chromatograms of fresh Rehmannia glutinosa formula granules, the characteristic chromatogram of iridoid glycosides in raw Rehmannia glutinosa glutinosa lacks a peak of 8, and the characteristic chromatogram of phenylethanol glycosides detects a peak of 9. These two characteristic peaks can be used for the exclusive identification of fresh and raw Rehmannia glutinosa formula granules; Injected the LPS for 2, 4, and 6 hours later, the high dose group of fresh Rehmannia glutinosa formula granules had a significant antipyretic effect (P＜0.05). The inhibition rate of temperature rise in rats with each formula granule sample was compared as follows: high dose of fresh Rehmannia glutinosa formula granules>high dose of raw Rehmannia glutinosa formula granules>low dose of fresh Rehmannia glutinosa formula granules>low dose of raw Rehmannia glutinosa formula granules. The antipyretic effect of fresh Rehmannia glutinosa formula granules on LPS induced fever model in rats was better. CONCLUSION There were differences in the characteristic chromatograms and antipyretic effects of fresh and raw Rehmannia glutinosa formula granules, and the antipyretic effect of fresh Rehmannia glutinosa formula granules was better. The research results can provide data support for the clinical promotion of fresh Rehmannia glutinosa formula granules. |
| Key words: Fresh Rehmannia glutinosa Raw Rehmannia glutinosa Formula Granules Iridoid glycosides Phenylethanol glycoside Characteristic chromatograms Antipyretic effect |
