| 引用本文: | 巴达拉胡,吴杰斯,包白音木其尔,刘小伟,敖道夫.基于Q-Exactive-Orbitrap的白豆蔻水提物及入血成分分析[1][J].中国现代应用药学,2025,42(9):22-30. |
| badalahu,wujiesi,baobaiyinmuqier,liuxiaowei,aodaofu.Analysis of Baidoukou Aqueous Extract and Blood Constituents Based on Q-Exactive-Orbitrap[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(9):22-30. |
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| 摘要: |
| 目的：拟采用UPLC-Q-Exactive-Orbitrap技术对白豆蔻水提物主要成分及大鼠口服入血成分进行分析。方法：运用ACQUITY UPLC HSS T3色谱柱（2.1×100 mm，1.8 μm）；以A（去离子水，含0.1%甲酸）和B（乙腈，含0.1%甲酸）为流动相，梯度洗脱，柱温：35℃；进样体积：10 μL；流速：0.2 mL/min。采用电喷雾离子源（ESI），Full ms/dd-ms2模式检测。结果：从白豆蔻水提物中共鉴定70种成分，包括有机酸类、黄酮类、氨基酸类、生物碱类、内酯类等。含药血清样本分析出19种原型成分，包括青蒿酸、壬二酸等11个有机酸类、异土木香内酯、去氢木香内酯等2种内酯类、甜菜碱、黄嘌呤等2种生物碱类、酪氨酸、色氨酸等3种氨基酸和1种其他类成分。结论：本研究首次阐明了白豆蔻水提物化学组成及口服入血成分，为其进一步研究和开发提供理论依据。 |
| 关键词: 白豆蔻 水提物 UPLC-Q-Exactive-Orbitrap 入血成分 |
| DOI：10.13748/j.cnki.issn1007-7693.20233045 |
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| Analysis of Baidoukou Aqueous Extract and Blood Constituents Based on Q-Exactive-Orbitrap |
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badalahu, wujiesi, baobaiyinmuqier, liuxiaowei, aodaofu
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Affiliated Hospital of Inner Mongolia Minzu University
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| Abstract: |
| OBJECTIVE: UPLC-Q-Exactive-Orbitrap technology is proposed to analyze the main components of Baidoukou aqueous extract and the components that enter the blood of rats after oral administration. METHODS: Using ACQUITY UPLC HSS T3 column (2.1×100 mm, 1.8 μm); Using A (deionized water, containing 0.1% formic acid) and B (acetonitrile, containing 0.1% formic acid) as mobile phases, gradient elution, column temperature: 35°C; injection volume: 10 μL; flow rate: 0.2 mL/min. Using electrospray ionization source (ESI), detect with Full ms/dd-ms2 mode. RESULTS: A total of 70 components were identified from the aqueous extract of Baidoukou, including organic acids, flavonoids, amino acids, alkaloids, and lactones. 19 prototype components were identified in the drug-containing serum samples, including 11 organic acids such as artemisinic acid and azelaic acid, 2 lactones such as isoalantolactone and dehydrocostus lactone, 2 alkaloids such as Betaine and Xanthine, 3 amino acids such as L-Tyrosine and L-Tryptophan, and 1 other component.CONCLUSIONS: It"s clarified for the first time the chemical composition of Baidoukou aqueous extract and its oral blood components in this study, providing a theoretical basis for its further research and development. |
| Key words: Baidoukou Aqueous Extract UPLC-Q-Exactive-Orbitrap Blood Constituents |
