| 引用本文: | 庄雪菲,李婷婷,李曦东,李惠芬,罗浩睿,潘林梅,范旻旻,程海波.基于肠吸收特征探讨“苦参-白花蛇舌草”药对的相互作用[J].中国现代应用药学,2025,42(9):13-21. |
| ZHUANG Xuefei,LI Tingting,LI Xidong,LI Huifen,LUO Haorui,PAN Linmei,FAN Minmin,CHEN Haibo.Study on Intestinal Absorption Characteristics and Interaction of Sophora flavescens and Hedyotis diffusaSWilld in Compound[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(9):13-21. |
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| 摘要: |
| 目的 本研究旨在探讨“苦参-白花蛇舌草”药对中有效成分在肠道中的吸收及相互作用特征。方法 通过构建大鼠肠外翻模型,采用高效液相色谱法检测肠吸收液中特征性成分苦参碱、氧化苦参碱和车叶草苷含量,计算其吸收参数,分析单味药与药对中成分在大鼠小肠不同部位、不同时间的吸收特征并对其肠吸收部位、时间、给药浓度进行研究。结果 通过对单味药、药对中的苦参碱、氧化苦参碱和车叶草苷吸收情况进行比较,发现相同吸收部位、相同浓度的样品溶液中,药对中苦参碱和车叶草苷的吸收量(Q)、吸收速率常数(Ka)较单药组显著增加(P＜0.05) 。同时,研究发现3种有效成分在十二指肠、空肠、回肠、结肠中均有一定的吸收,在回肠中得到累计吸收量(Q)最高。随时间与浓度的增加,3种有效成分在肠道中吸收量(Q)也随之增加。结论 “苦参-白花蛇舌草”药对配伍应用产生协同增效的治疗作用,这3种成分肠吸收特性可能为具有时间与浓度依赖性的被动运输模式且在回肠段中吸收最佳,该研究结果可以为进一步的临床用药设计提供参考。 |
| 关键词: “苦参-白花蛇舌草”药对 氧化苦参碱 苦参碱 车叶草苷 离体肠外翻 肠吸收 |
| DOI： |
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| 基金项目:国家重点研发计划(No.2017YFC1700602) |
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| Study on Intestinal Absorption Characteristics and Interaction of Sophora flavescens and Hedyotis diffusaSWilld in Compound |
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ZHUANG Xuefei1, LI Tingting1, LI Xidong1, LI Huifen1, LUO Haorui1, PAN Linmei1, FAN Minmin2, CHEN Haibo2
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1.School of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023, China;2.The First Clinical College of Nanjing University of Chinese Medicine, Nanjing 210023, China
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| Abstract: |
| ABSTRACT: OBJECTIVE This study aims to investigate the absorption characteristics and interaction of active ingredients of Sophora flavescent and Hedyotis diffusa Willd individual herbs and drug pair in intestine. METHODS The location, time and concentration of the intestinal absorption were studied by everted intestinal sac models. The contents of matrine, oxymatrine and asperuloside in the intestinal sac absorption solution were detected by high performance liquid chromatography (HPLC), and the absorption kinetic parameters (Q and Ka) were calculated to investigate the intestinal absorption characteristics of each component. RESULTS The three active components were detected in duodenum, jejunum, ileum and colon, and the cumulative absorption (Q) was the highest in the ileum. With the increase of time and concentration, the absorption (Q) of the three active ingredients in the intestine also increased. The co-extraction of sophora flavescens and Hedyotis diffusa Willd could promote the intestinal absorption of matrine and luciferin. CONCLUSION Sophora - Hedyotis diffusa Willd herbal pair has a synergistic therapeutic effect on the compatible application, and the intestinal absorption characteristics of these three components may be passive transport mode with time and concentration dependence and the best absorption in the ileum segment. |
| Key words: Oxymatrine Matrine Sophora - Hedyotis diffusa Willd herbal pair Asperuloside Everted intestinal sac models |
