| 引用本文: | 朱义越,陆晔晗,常芮宁,宋艺,王艾雯,王春丽,郭美珠,张春芳.白花蛇舌草总多酚纯化工艺及体外抗炎活性研究[J].中国现代应用药学,2025,42(7):1-8. |
| zhuyiyue,luyehan,changruining,songyi,wangyiwen,wangchunli,guomeizhu,zhangchunfang.Purification process and in vitro anti-inflammatory activity of total polyphenols from Hedyotis diffusa Willd.[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(7):1-8. |
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| 摘要: |
| 摘要：目的 探讨大孔树脂对于白花蛇舌草总多酚的最佳纯化工艺条件及其体外抗炎效果。方法 采用超声波辅助提取法提取白花蛇舌草总多酚，静态吸附-解吸平衡动力学实验研究不同大孔树脂吸附效果，筛选最佳纯化工艺条件，并通过5-脂氧合酶和环氧合酶抑制实验评价其抗炎活性。结果 采用HPD-826型大孔树脂纯化白花蛇舌草总多酚在上样液浓度为1.5mg/mL、pH=5.0、上样体积90 mL、上样流速为1.0 mL/min、洗脱液乙醇体积分数为70%、洗脱剂体积80 mL、洗脱流速为1.0 mL/min条件下纯化效果最好，白花蛇舌草总多酚纯度由27.13 ± 1.36% 提升到63.08 ± 0.83%，提高了1.32倍。总多酚浓度为20 mg/mL的白花蛇舌草醇提液，对5-LOX活性抑制率可达到57.15 ± 7.16%；总多酚浓度为3.2 mg/mL的白花蛇舌草醇提液对COX-2活性抑制率可达到83.49 ± 2.48%。结论 大孔树脂纯化技术可以提高白花蛇舌草总多酚的纯度，其对炎症介导的5-LOX和COX-2均具有抑制作用，且对COX-2的抑制效果更佳。 |
| 关键词: 白花蛇舌草 大孔树脂 多酚 体外抗炎 |
| DOI： |
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| 基金项目:上海市宝山区科委项目 |
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| Purification process and in vitro anti-inflammatory activity of total polyphenols from Hedyotis diffusa Willd. |
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zhuyiyue1, luyehan1, changruining1, songyi1, wangyiwen1, wangchunli1, guomeizhu2, zhangchunfang3
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1.School of Pharmacy, East China University of Science and Technology;2.Shanghai Baoshan District Hospital of Integrated Chinese and Western Medicine Shanghai;3.Xiang ’an Hospital of Xiamen University, School of Medicine, Xiamen University
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| Abstract: |
| ABSTRACT: OBJECTIVE To study the purification effect of macroporous resin on the total polyphenolic compounds of Hedyotis diffusa and to investigate the optimal purification process conditions, and to investigate the in vitro anti-inflammatory effect of Hedyotis diffusa. METHODS The total polyphenols in Hedyotis diffusa were extracted by ultrasound-assisted extraction, and the adsorption effect of different macroporous resins was investigated in static adsorption-desorption equilibrium kinetic experiments to screen the optimal purification process conditions, and the anti-inflammatory activity was evaluated by the inhibition of 5-lipoxygenase and cyclooxygenase assays. RESULTS The purification of total polyphenols from Hedyotis diffusa using HPD-826 macroporous resin was found to be most effective under the following conditions: sample concentration of 1.5 mg/mL, pH of 5.0, sample volume of 90 mL, sample flow rate of 1.0 mL/min, elution solution ethanol volume fraction of 70%, elution solution volume of 80 mL, and elution flow rate of 1.0 mL/min. Under these conditions, the purity of total polyphenols from Hedyotis diffusa increased from 27.13 ± 1.36% to 63.08 ± 0.83%, representing a 1.32-fold improvement. The ethanolic extract of Hedyotis diffusa with a total polyphenol concentration of 20 mg/mL exhibited a 57.15 ± 7.16% inhibition rate against 5-lipoxygenase (5-LOX) activity, while the ethanolic extract with a total polyphenol concentration of 3.2 mg/mL demonstrated an 83.49 ± 2.48% inhibition rate against cyclooxygenase-2 (COX-2) activity. CONCLUSION The macroporous resin purification technology can enhance the purity of total polyphenols from Hedyotis diffusa. It exhibits inhibitory effects on inflammation-mediated 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2), with a more pronounced inhibitory effect on COX-2.
KEY WORDS: Hedyotis diffusa Willd; macroporous resin; polyphenols vitro anti-inflammatory |
| Key words: Hedyotis diffusa Willd macroporous resin polyphenols vitro anti-inflammatory |
