| 引用本文: | 李楠楠,孟宪生,郭文昭,薛立平,卢秉久.基于RhoA/ROCK通路探讨软肝冲剂对CCl4所致大鼠肝纤维化的作用机制[J].中国现代应用药学,2024,41(16):69-75. |
| LI nan nan,Meng xiansheng,GUO wenzhao,XUE liping,LU bingjiu.Effects of Ruanganchongji on CCl4-induced Hepatic Fibrosis in Rats Based on RhoA/ROCK Pathway[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(16):69-75. |
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| 摘要: |
| 目的 从RhoA/ROCK通路角度探究软肝冲剂对CCl4所致大鼠肝纤维化的分子机制。方法 灌胃给与2 mL·kg-1 40% CCl4(CCl4:橄榄油=2:3,v:v)每周2次,共计8周,建立大鼠肝纤维化模型。造模第6周给与软肝冲剂干预8周,采用Masson染色、脏器指数、生化指标评价软肝冲剂对CCl4所致大鼠肝纤维化的作用；采用RT-PCR、Western blot及免疫荧光组织化学法检测大鼠肝脏组织中Coll Ⅰ、α-SMA、RhoA、ROCK 1 mRNA和蛋白质的表达。结果 脏器指数和生化指标结果显示,与模型组比较,软肝冲剂可以不同程度降低大鼠的肝指数、脾指数,升高胸腺指数(P<0.05,P<0.01)；软肝冲剂可以不同程度降低大鼠的血清中AFP、ALT、AST水平(P<0.05,P<0.01)；Masson染色结果显示,软肝冲剂可以显著降低大鼠肝脏胶原纤维增生；RT-PCR、免疫荧光组织化学和Western blot结果显示,软肝冲剂可以显著降低大鼠肝脏组织中Coll Ⅰ、α-SMA、RhoA、ROCK 1 mRNA和蛋白质的表达。结论 软肝冲剂对CCl4所致大鼠肝纤维化的治疗作用可能与抑制RhoA/ROCK通路有关。 |
| 关键词: 软肝冲剂 肝纤维化 RhoA/ROCK通路 作用机制 |
| DOI：10.13748/j.cnki.issn1007-7693.20231905 |
| 分类号: |
| 基金项目:辽宁省重点研发计划项目(2020JH2/10300066)；辽宁省自然科学基金计划项目(2022-BS-200)。 |
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| Effects of Ruanganchongji on CCl4-induced Hepatic Fibrosis in Rats Based on RhoA/ROCK Pathway |
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LI nan nan1, Meng xiansheng1, GUO wenzhao1, XUE liping2, LU bingjiu2
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1.Liaoning University of Traditional Chinese Medicine;2.Liaoning Traditional Chinese Medicine
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| Abstract: |
| ABSTRACT: OBJECTIVE Based on RhoA/ROCK pathway to explore the mechanism of Ruanganchongji against CCl4-induced hepatic fibrosis in rats. METHODS The rat hepatic fibrosis model was established by intragastric administration of 40% CCl4 (2 mL·kg-1), twice a week for 8 weeks. After the intervention of Ruanganchongji at 6 week for 8 weeks, masson staining, organ index and biochemical indexes to evaluate the effect of Ruanganchongji on CCl4-induced hepatic fibrosis in rats. RT-PCR, Western Blot and immunofluorescent tissue chemistry was used to detected the expression of Coll Ⅰ, α-SMA, RhoA, ROCK 1 mRNA and protein in rats. RESULTS The organ index and biochemical analysis indicated that, compared to the model control group, Ruanganchongji can reduce the liver index, spleen index and increase the thymus index in different degrees (P<0.05, P<0.01); Ruanganchongji can reduce the levels of AFP, ALT and AST in the serum; The results of Masson staining showed that Ruanganchongji could significantly reduce the proliferation of collagen fibers in rat liver; RT-PCR,Western blot and Immune fluorescent tissue chemistry results showed that Ruanganchongji could significantly downregulate the expression of Coll Ⅰ, α-SMA, RhoA, and ROCK 1 mRNA and protein. CONCLUSION The therapeutic effect of Ruanganchongji on CCl4-induced hepatic fibrosis in rats may be related to the inhibition of RhoA/ROCK pathway. |
| Key words: ruanganchongji hepatic fibrosis RhoA/ROCK pathway mechanism |
