| 引用本文: | 钟丽丽,秦元锴,刘玫,赵霞.多索茶碱通过激活 AMPK 通路改善过氧化氢诱导的PC12细胞氧化损伤[J].中国现代应用药学,2024,41(15):6-11. |
| ZHONG Lili,QIN Yuankai,LIU Mei,ZHAO Xia.Doxofylline attenuates hydrogen peroxide-induced oxidative damage in PC12 cells by activating AMPK pathway[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(15):6-11. |
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| 摘要: |
| 目的 研究多索茶碱对过氧化氢 (H2O2) 诱导的PC12细胞毒性的保护作用及其潜在机制。方法 采用 H2O2 建立细胞氧化损伤模型。MTT检测细胞活力；Tunel染色、流式细胞仪、Caspase3活性试剂盒检测细胞凋亡；乳酸脱氢酶（LDH）试剂盒检测细胞膜损伤；线粒体膜电位检测试剂盒（JC-1）和DCFH-DA试剂检测线粒体功能变化；蛋白质免疫印迹用于检测信号通路蛋白水平的变化。结果 多索茶碱浓度依赖性改善了 H2O2诱导的PC12细胞内活性氧（ROS）的异常水平，恢复了线粒体膜电位，降低了Caspase-3激活，进而降低了PC12细胞凋亡。多索茶碱还以时间和浓度依赖性方式刺激 PC12 细胞中AMPK的磷酸化，其保护作用可被AMPK通路抑制剂逆转。结论 多索茶碱通过激活AMPK信号通路改善H2O2诱导的PC12细胞损伤。 |
| 关键词: 多索茶碱 过氧化氢 PC12细胞 氧化应激 AMPK信号通路 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目:浙江省自然科学基金(Q23H090045)，杭州医学院人才启动项目（00004F1RCYJ2109） |
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| Doxofylline attenuates hydrogen peroxide-induced oxidative damage in PC12 cells by activating AMPK pathway |
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ZHONG Lili, QIN Yuankai, LIU Mei, ZHAO Xia
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Hangzhou Medical College
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| Abstract: |
| OBJECTIVE To investigate the protective effect of doxofylline against hydrogen peroxide (H2O2)-induced cytotoxicity in PC12 and its underlying mechanism. METHODS The cell oxidative damage model was established by H2O2. MTT assay was used to detect the cell viability; Tunel staining, flow cytometry and caspase3 activation kit were used to detect cell apoptosis; lactate dehydrogenase kit to detect cell membrane damage; mitochondrial membrane potential detection kit (JC-1) and DCFH-DA reagent were used to detect changes in mitochondrial function; Western blotting was used to detect changes in signaling pathway protein levels. RESULTS Doxofylline concentration-dependently improved H2O2-induced abnormal levels of reactive oxygen species (ROS) in PC12 cells, restored mitochondrial membrane potential, reduced Caspase-3 activation, and finally reduced PC12 cell apoptosis. Doxofylline also stimulated phosphorylation of AMPK in PC12 cells in a time- and concentration-dependent manner, and its protective effect was reversed by AMPK pathway inhibitors. CONCLUSION Doxofylline improves H2O2-induced PC12 cell injury by activating AMPK signaling pathway. |
| Key words: doxofylline hydrogen peroxide PC12 cells oxidative stress AMPK signaling pathway |
