| 引用本文: | 汤慧敏,付静,熊乐文,张龙霏,张永清,王玲娜.激光显微切割技术联合HPLC-TOF/MS定性分析金银花花蕾腺毛成分[J].中国现代应用药学,2023,40(18):2499-2505. |
| TANG Huimin,FU Jing,XIONG Lewen,ZHANG Longfei,ZHANG Yongqing,WANG Lingna.Qualitative Analysis of Components of Glandular Hairs in Lonicera Japonica Thunb. Buds by Laser Microdissection Combined with HPLC-TOF/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(18):2499-2505. |
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| 摘要: |
| 目的 分析金银花花蕾腺毛成分，为金银花药材质量评价和控制提供参考。方法 采用激光显微切割技术获取花蕾腺毛，低温冷冻涡旋法去除花蕾表皮毛（腺毛与非腺毛），HPLC-TOF/MS检测花蕾、去表皮毛花蕾和腺毛中的成分，通过对照品对照、数据库检索及质谱裂解规律进行结构解析和鉴定。结果 从花蕾中鉴定成分37种，去表皮毛花蕾中鉴定31种，腺毛中鉴定35种；相对于花蕾，腺毛缺少2种酚酸类成分，去表皮毛花蕾缺少6种黄酮类成分。结论 腺毛含有多种次生物质，是金银花花蕾次生物质合成积累的重要场所，与金银花药材品质密切相关。 |
| 关键词: 金银花 腺毛 激光显微切割技术 HPLC-TOF/MS 成分 |
| DOI：10.13748/j.cnki.issn1007-7693.20230022 |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目（81903750）；山东省自然科学基金项目（ZR2021QH238）；中央本级重大增减支项目（2060302）；山东省重大科技创新工程（2019JZZY011020） |
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| Qualitative Analysis of Components of Glandular Hairs in Lonicera Japonica Thunb. Buds by Laser Microdissection Combined with HPLC-TOF/MS |
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TANG Huimin, FU Jing, XIONG Lewen, ZHANG Longfei, ZHANG Yongqing, WANG Lingna
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Shandong University of Traditional Chinese Medicine, Jinan 250355, China
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| Abstract: |
| OBJECTIVE To accurately analyze the components of glandular hairs in Lonicera japonica Thunb. buds, and provide reference for quality evaluation and control of Lonicera japonica Thunb.. METHODS Laser microdissection technology was used to obtain glandular hairs of Lonicera japonica Thunb. buds, low-temperature freezing vortex method was used to removal flower buds surface hairs(glandular hairs and non glandular hairs), and the components of flower buds, flower buds removed surface hairs and glandular hairs were detected by HPLC-TOF/MS. Through comparison with standards, database retrieval and mass spectrometry fragmentation, the components were analyzed and identified. RESULTS Thirty-seven components were identified in the flower buds, 31 components were identified in the flower buds removed surface hairs, and 35 components were identified in the glandular hairs. Compared with flower buds, glandular hairs lack 2 phenolic acids, and flower buds removed surface hairs lack 6 flavonoids. CONCLUSION The glandular hair, which contains many secondary substances, is an important place for the synthesis and accumulation of secondary substances in Lonicera japonica Thunb. buds, and is closely related to the quality of Lonicera japonica Thunb.. |
| Key words: Lonicera japonica Thunb. glandular hair laser microdissection technology HPLC-TOF/MS component |
