| 引用本文: | 宋现良,王香君,夏小雯,任亚爽,郭德第,宋春红.利用UHPLC-MS/MS靶标代谢组学研究柴芍皂苷对PMDD肝气郁证模型大鼠海马脑区神经递质的影响[J].中国现代应用药学,2024,41(12):16-23. |
| Song Xianliang,Wang Xiangjun,Xia Xiaowen,Ren Yashuang,Guo Dedi,Song Chunhong.Effect of Chai Shao Saponin on neurotransmitters in the hippocampal brain region of rats with PMDD model of liver qi depression using UHPLC-MS/MS target metabolomics[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(12):16-23. |
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| 摘要: |
| 目的 研究柴芍皂苷对PMDD肝气郁证模型大鼠的治疗作用及对海马脑区神经递质的影响。方法 通过择时慢性束缚应激法制备PMDD肝气郁证大鼠模型，柴芍皂苷干预，旷场实验和强迫游泳实验评价大鼠抑郁症状，运用UHPLC-MS/MS靶标代谢组学检测大鼠海马脑区神经递质水平变化。结果 柴芍皂苷明显改善PMDD肝气郁证大鼠的抑郁症状，PMDD大鼠海马脑区5-HT、Glu、GABA、NE水平均显著升高，柴芍皂苷干预后5-HT、Glu、GABA、NE、DA和E水平均与正常组没有差异。结论 柴芍皂苷通过调节神经递质水平缓解PMDD肝气郁证大鼠抑郁症状，柴芍皂苷具有开发为治疗PMDD肝气郁证新药的潜力，调节神经递质水平可能是柴胡、白芍类方剂临床治疗PMDD肝气郁证的作用途径。 |
| 关键词: 柴芍皂苷 PMDD肝气郁证 神经递质 |
| DOI：10.13748/j.cnki.issn1007-7693.20224251 |
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| 基金项目:山东省自然科学基金面上项目(ZR2020MH341) |
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| Effect of Chai Shao Saponin on neurotransmitters in the hippocampal brain region of rats with PMDD model of liver qi depression using UHPLC-MS/MS target metabolomics |
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Song Xianliang1, Wang Xiangjun2, Xia Xiaowen2, Ren Yashuang1, Guo Dedi1, Song Chunhong2
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1.College of Pharmacy, Shandong University of Traditional Chinese Medicine;2.Central Hospital Affiliated to Shandong First Medical University
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| Abstract: |
| OBJECTIVE Study on the therapeutic effect of Chai Shao Saponin on rats with PMDD model of liver qi depression and the effect on neurotransmitters in hippocampal brain area. METHODS The PMDD rat model of liver qi depression was prepared by the time-chosen chronic restraint stress method, and the depressive symptoms of rats were evaluated by the intervention of Chai Shao Saponin, the open field experiment and forced swimming experiment, and the changes of neurotransmitter levels in the hippocampal brain region of rats were detected by UHPLC-MS/MS target metabolomics. RESULTS The levels of 5-HT, Glu, GABA and NE in the hippocampal brain area of PMDD rats were significantly increased, and the levels of 5-HT, Glu, GABA, NE, DA and E were not different from those of the normal group after the intervention of Chai Shao Saponin. CONCLUSION Chai Shao Saponin alleviates depression in rats with PMDD by regulating neurotransmitter levels, Chai Shao Saponin has the potential to be developed as a new drug for the treatment of PMDD with liver qi depression, and regulating neurotransmitter levels may be the pathway for the clinical treatment of PMDD with liver qi depression in the formulations of Chai Hu and Bai Shao. |
| Key words: chai shao Saponin, PMDD liver qi depression evidence neurotransmitters |
