| 引用本文: | 景秀村,李军茂,冯育林,葛菲,姚闽,白吉庆.基于UHPLC-Q-TOF-MS/MS分析猪牙皂中化学成分[J].中国现代应用药学,2023,40(23):3225-3235. |
| JING Xiucun,LI Junmao,FENG Yulin,GE Fei,YAO Min,BAI Jiqing.Analysis of Chemical Constituents in Gleditsiae Fructus Abnormalis Based on UHPLC-Q-TOF-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(23):3225-3235. |
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| 基于UHPLC-Q-TOF-MS/MS分析猪牙皂中化学成分 |
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景秀村1, 李军茂1, 冯育林1, 葛菲1, 姚闽2, 白吉庆1,3,4
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1.江西中医药大学, 南昌 330004;2.江西省药品检验检测研究院, 南昌 330004;3.陕西中医药大学, 陕西 咸阳 712046;4.陕西省中药原料质量检测技术服务中心 陕西 咸阳 712046
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| 摘要: |
| 目的 建立液质联用分析方法全面、快速识别猪牙皂中的化学成分。方法 应用超高液相色谱-四极杆飞行时间串联质谱联用技术(UHPLC-Q-TOF-MS/MS)对猪牙皂提取物进行成分分析；其流动相为0.1%甲酸(A)-乙腈(B)，梯度洗脱，流速0.3 mL·min-1。在负离子模式下获得精确的分子离子质量及二级碎片信息，结合数据库及对照品进行结构鉴定。结果 共筛选出78种化合物(黄酮类化合物32个，酚酸类化合物15个，木脂素类22个，单萜酸类化学成分9个)，潜在新化合物14个。结论 本研究全面分析了猪牙皂的化学成分，为该药物药效物质基础和质量标准的研究提供了依据。 |
| 关键词: 猪牙皂 皂荚 化学成分 UHPLC-Q-TOF-MS/MS |
| DOI：10.13748/j.cnki.issn1007-7693.20223494 |
| 分类号:R284.1 |
| 基金项目:江西省中医药中青年骨干人才培养计划项目[赣中医药科教字(2020)2号] |
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| Analysis of Chemical Constituents in Gleditsiae Fructus Abnormalis Based on UHPLC-Q-TOF-MS/MS |
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JING Xiucun1, LI Junmao1, FENG Yulin1, GE Fei1, YAO Min2, BAI Jiqing1,3,4
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1.Jiangxi University of Chinese Medicine, Nanchang 330004, China;2.Jiangxi Institute for Drug Control, Nanchang 330004, China;3.Shaanxi University of Chinese Medicine, Xianyang 712046, China;4.Shaanxi Chinese Medicine Raw Material Quality Testing Technology Service Center, Xianyang 712046, China
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| Abstract: |
| OBJECTIVE To Establish a liquid chromatography-mass spectrometry analysis method to comprehensively and quickly identify the chemical components in Gleditsiae Fructus Abnormalis. METHODS The ultra-high liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS) was applied for the composition analysis of Gleditsiae Fructus Abnormalis extract; its mobile phase was 0.1% formic acid(A)-acetonitrile(B) with gradient elution at a flow rate of 0.3 mL·min-1. Accurate ion mass and secondary fragmentation information were obtained in negative ion mode, combined with databases and standard reference for structure elucidation. RESULTS A total of 78 compounds(32 flavonoids, 15 phenolic acids, 22 lignans and 9 chemical constituents of monoterpenoid acids) with 14 potential new compounds were screened. CONCLUSION This study comprehensively analyzes the chemical composition of Gleditsiae Fructus Abnormalis, and provides a basis for the investigation of the pharmacodynamic basis and quality standards of this drug. |
| Key words: Gleditsiae Fructus Abnormalis zleditsia sinensis chemical composition UHPLC-Q-TOF-MS/MS |
