| 引用本文: | 张晓芹,林炳锋,刘爽,雷后兴,陈礼平,王娜妮.基于UHPLC-Q/TOF-MS技术鉴定青钱柳叶在大鼠体内的入血成分及代谢产物[J].中国现代应用药学,2023,40(17):2435-2445. |
| ZHANG Xiaoqin,LIN Bingfeng,LIU Shuang,LEI Houxing,CHEN Liping,WANG Nani.Identification of Components and Metabolites of Leaves of Cyclocarya Paliurus in Rats Plasma by UHPLC-Q/TOF-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(17):2435-2445. |
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| 摘要: |
| 目的 研究青钱柳叶在大鼠体内的入血成分及代谢产物。方法 采用超高效液相色谱-飞行时间质谱联用技术(UHPLC-Q/TOF-MS)鉴定大鼠血清中的化学成分，通过比对青钱柳叶、空白血清和含药血清的图谱差异，解析青钱柳水提液经大鼠灌胃后血清中的原型成分和代谢产物。结果 共鉴定得到15个入血成分，6个为原型成分，9个为原型成分的代谢产物。入血的原型成分主要为咖啡酰奎宁酸类、黄酮类和皂苷类，代谢途径主要有甲基化和羟基化。结论 该研究表明入血的原型成分可能是青钱柳叶的有效成分，为阐明其药效物质基础提供了一定的依据。 |
| 关键词: 青钱柳 血清药物化学 原型成分 代谢产物 UHPLC-Q/TOF-MS |
| DOI：10.13748/j.cnki.issn1007-7693.20222841 |
| 分类号:R969.1 |
| 基金项目:丽水市公益项目(2021GYX18)；丽水市畲医药学重点学科 |
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| Identification of Components and Metabolites of Leaves of Cyclocarya Paliurus in Rats Plasma by UHPLC-Q/TOF-MS |
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ZHANG Xiaoqin1, LIN Bingfeng2, LIU Shuang1, LEI Houxing1, CHEN Liping1, WANG Nani2
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1.Lishui Hospital of Traditional Chinese Medicine, Lishui 323000, China;2.Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China
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| Abstract: |
| OBJECTIVE To investigate the components and metabolites of leaves of Cyclocarya paliurus in rats plasma. METHODS UHPLC-Q/TOF-MS method was applied to identify the components and metabolites of rat plasma. The prototype components and metabolites were analyzed in rats plasma of leaves of Cyclocarya paliurus water extract after intragastric administration by comparing the chromatograms of Cyclocarya paliurus, blank serum and drug-containing serum. RESULTS A total of 15 compounds including 6 prototype components and 9 metabolites were identified. The main prototype components in blood were caffeylquinic acid, flavonoids and saponins, and the metabolic pathways were methylation and hydroxylation. CONCLUSION This experiment showes that the prototype components absorbed into blood may be the potential bioactive components in leaves of Cyclocarya paliurus, providing a scientific basis for clarifying its material basis in pharmacodynamics. |
| Key words: Cyclocarya paliurus serum pharmacochemistry prototype component metabolites UHPLC-Q/TOF-MS |
