| 引用本文: | 袁铭铭,熊晓丽,代敏,钟瑞建,吴良发.HPLC同时测定大青叶中2个专属性酚酸的含量[J].中国现代应用药学,2023,40(14):1963-1966. |
| YUAN Mingming,XIONG Xiaoli,DAI Min,ZHONG Ruijian,WU Liangfa.Simultaneous Determination of 2 Specific Phenolic Acid in Isatidis Folium by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(14):1963-1966. |
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| 摘要: |
| 目的 建立同时测定大青叶中1,2,2'-tri-O-E-sinapoyl-β-gentiobiose和1,2,6'-tri-O-E-sinapoylgentiobiose 2个专属性酚酸的含量方法。方法 采用依利特C18色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-0.1%磷酸溶液为流动相，梯度洗脱，流速为1.0 mL·min-1，检测波长328 nm，柱温30 ℃。结果 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose和1,2,6'-tri-O-E- sinapoylgentiobiose分别在2.092~104.6 μg·mL-1(R2=0.999 6)和2.996~149.8 μg·mL-1(R2=0.999 5)内呈现出良好的线性关系；精密度、稳定性和重复性的RSD均<2%；平均加样回收率(n=6)分别为99.05%，99.60%，RSD分别为0.34%，0.65%。结论 该方法简便，专属性、重复性好，可用于测定大青叶2个专属性酚酸的含量测定方法。 |
| 关键词: 大青叶 高效液相色谱法 酚酸类 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.20222333 |
| 分类号:R917.101 |
| 基金项目:江西省重点研发计划项目(20203BBGL73215) |
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| Simultaneous Determination of 2 Specific Phenolic Acid in Isatidis Folium by HPLC |
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YUAN Mingming, XIONG Xiaoli, DAI Min, ZHONG Ruijian, WU Liangfa
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Jiangxi Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Jiangxi Province Engineering Research Center of Drug and Medical Device Quality, Nanchang 330029, China
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| Abstract: |
| OBJECTIVE To establish a method for the simultaneous determination of 2 specific phenolic acids glycosides 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose in Isatidis Folium. METHODS The HPLC analysis was carried on Elite C18 column(4.6 mm×250 mm, 5 μm) with mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution(gradient elution) at the flow rate of 1.0 mL·min-1. The detection wavelength was 328 nm, and the column temperature was set at 30 ℃. RESULTS 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose were showed good linear relationships within the ranges of 2.092-104.6 μg·mL-1(R2=0.999 6) and 2.996-149.8 μg·mL-1(R2=0.999 5). The average recovery (n=6) were 99.05% and 99.60%, RSDs were 0.34% and 0.65%, respectively. CONCLUSION The established method is simple, reliable and specific, and suitable for simultaneous determination of two components of phenolic acids glycosides in Isatidis Folium. |
| Key words: Isatidis Folium HPLC phenolic acids content determination |
