| 引用本文: | 汪丽霞,黎思慧,马奕涵,吴斌.海南红树林内生真菌Fusarium sp. HSL-3次级代谢产物研究[J].中国现代应用药学,2023,40(13):1759-1764. |
| WANG Lixia,LI Sihui,MA Yihan,WU Bin.Study on Secondary Metabolites of Endophytic Fungus Fusarium sp. HSL-3 from Hainan Mangrove[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(13):1759-1764. |
|
| 摘要: |
| 目的 研究海南红树林内生真菌Fusarium sp. HSL-3次级代谢产物,并对其进行抗炎和抗肿瘤活性筛选。方法 采用大米培养基对菌株静置培养,同时采用柱层析色谱法和HPLC对该真菌的次级代谢产物进行分离纯化,并且通过核磁共振及质谱等有机波谱手段,对得到的化合物进行结构鉴定。结果 共分离得到8个化合物,分别为lateritin(1)、4-carbomethoxy-6-hydroxy-2-quinolone(2)、3,5-dimethoxydihydrofusarubin D(3)、anhydrofusarbin(4)、3,3'-methylene-bis (4-hydroxybenzaldehyde)(5)、crypticin B(6)、vanillyl alcohol(7)和3,4-dihydroxyphenylaceticacid(8),其中化合物2和5为首次从镰刀菌属Fusarium中分离得到。化合物3和5对RAW264.7细胞表现出良好的抗炎活性(化合物3在50 µmol·L-1时的NO抑制率为87%,化合物5在50 µmol·L-1时的NO抑制率为71%),而化合物1对非小细胞肺癌细胞A549有显著的细胞毒活性,IC50值为(7.92±0.27)µmol·L-1。结论 从海南红树林内生真菌Fusarium sp. HSL-3中分离得到的化合物3和5具有较强的抗炎活性,化合物1有显著的抗肿瘤活性。 |
| 关键词: 红树林 内生真菌 Fusarium sp 次级代谢产物 抗炎 抗肿瘤 |
| DOI：10.13748/j.cnki.issn1007-7693.20222302 |
| 分类号:R927 |
| 基金项目:国家自然科学基金项目(42176098，41876148)；常山县科技研发和成果转化项目(2021026) |
|
| Study on Secondary Metabolites of Endophytic Fungus Fusarium sp. HSL-3 from Hainan Mangrove |
|
WANG Lixia1,2, LI Sihui2, MA Yihan2, WU Bin2
|
|
1.Changshan Agriculture Development Center, Changshan 324200, China;2.Ocean College, Zhejiang University, Hangzhou 310000, China
|
| Abstract: |
| OBJECTIVE To study the secondary metabolites of the endophytic fungus Fusarium sp. HSL-3 isolated from Hainan Mangrove and screen their anti-inflammatory and anti-tumor activities. METHODS This fungus was fermented statically by using rice medium, and the secondary metabolites of the fungus were isolated and purified by column chromatography and HPLC. Structures indentification of the ortained compounds was carried out through MS and NMR. RESULTS Eight compounds were respectively isolated and identified as follows:lateritin(1), 4-carbomethoxy-6-hydroxy- 2-quinolone(2), 3,5-dimethoxydihydro-fusarubin D(3), anhydrofusarbin(4), 3,3'-methylene-bis(4-hydroxybenzaldehyde)(5), crypticin B(6), vanillyl alcohol(7) and 3,4-dihydroxyphenylaceticacid(8), among which, compounds 2 and 5 were isolated from the genus Fusarium for the first time. Compounds 3 and 5 showed good anti-inflammatory activity on RAW264.7 cells with the inhibition rate of NO at 50 µmol·L-1 were 87% for compound 3 and 71% for compound 5, while compound 1 showed significant cytotoxic activity against human non-small cell lung cancer cell line A549, among which the IC50 value was (7.92±0.27) µmol·L-1. CONCLUSION Compounds 3 and 5 isolated from endophytic fungus Fusarium sp. HSL-3 isolated from Hainan Mangrove show strong anti-inflammatory activity, while compound 1 shows significant anti-tumor activity. |
| Key words: Mangrove endophytic fungus Fusarium sp. secondary metabolites anti-inflammatory anti-tumor |
