| 引用本文: | 王芳宇,孙浩,徐英,梁颖,张慧杰.肾衰方汤剂的去钾工艺研究[J].中国现代应用药学,2023,40(16):2243-2250. |
| WANG Fangyu,SUN Hao,XU Ying,LIANG Ying,ZHANG Huijie.Study of Removing Potassium from Shenshuai Recipe[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(16):2243-2250. |
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| 肾衰方汤剂的去钾工艺研究 |
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王芳宇1,2, 孙浩1,2, 徐英3, 梁颖1,2, 张慧杰1,2
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1.天津中医药研究院附属医院药学部, 天津 300120;2.天津中医药研究院附属医院中药研究所, 天津 300120;3.天津中医药研究院附属医院肾病科, 天津 300120
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| 摘要: |
| 目的 采用药用阳离子交换树脂(聚磺苯乙烯钠)在体外进行中药汤剂“肾衰方”中K+的去除研究，建立中药汤剂的体外去除K+工艺。方法 采用电感耦合等离子体质谱法测定汤剂中的K+和Na+浓度，采用超高效液相色谱测定汤剂中各指标成分含量的变化。经树脂性能的考察，确定采用阳离子树脂静态吸附法，并初步判定K+去除的影响因素有温度、树脂用量、吸附时间。采用单因素试验研究这3个因素对水煎液中K+吸附的影响。结果 最佳工艺条件：温度为25 ℃，树脂用量为汤剂∶树脂(15 mL∶1 g)，吸附时间为1 min。本工艺应用于中药“肾衰方”K+的去除率>80%，应用于临床不同肾病处方的K+去除率为62.5%~80.3%，极大地降低了肾衰患者服用中药汤剂K+的摄入。研究发现此药用树脂对K+的吸附作用受离子环境的影响较大，对各指标成分的吸附也具有一定的选择性。结论 建立的去钾工艺能够很好地去除“肾衰方”汤剂中的K+。 |
| 关键词: 肾衰方 钾离子 阳离子交换树脂 聚磺苯乙烯钠 静态吸附 |
| DOI：10.13748/j.cnki.issn1007-7693.20222245 |
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| 基金项目:天津市卫生健康委员会、天津市中医药管理局中医中西医结合科研课题(2019025，2021182)；天津市中医药研究院附属医院院内培育基金项目(2020003) |
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| Study of Removing Potassium from Shenshuai Recipe |
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WANG Fangyu1,2, SUN Hao1,2, XU Ying3, LIANG Ying1,2, ZHANG Huijie1,2
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1.Hospital Affiliated to Tianjin Academy of Traditional Chinese Medicine, Department of Pharmacy, Tianjin 300120, China;2.Hospital Affiliated to Tianjin Academy of Traditional Chinese Medicine, Chinese Medicine Research Institute, Tianjin 300120, China;3.Hospital Affiliated to Tianjin Academy of Traditional Chinese Medicine, Department of Nephrology, Tianjin 300120, China
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| Abstract: |
| OBJECTIVE To conduct the removal of K+ in traditional Chinese medicine decoction of Shenshuai Recipe by using the medicinal cation exchange resin(sodium polystyrene sulfonate). METHODS The concentration of K+ and Na+ in the decoction were determined by the ICP-MS method. And the UPLC method was established to determined the impact of K+ removal technique on indicative compounds in the decoction. After investigating the resin properties, static adsorption of cation exchange resin was applied in the study. It was preliminarily determined that the factors affecting K+ removal was temperature, resin amount and adsorption time. The single factor experiment was conducted to investigated the influence of these three factors on K+ adsorption in decoction. RESULTS The appropriate process conditions were determined as follows: the temperature was 25 ℃, the amount of resin was decoction︰resin (15 mL︰1 g), and the adsorption time was 1 min. The removal rate of K+ was > 80% in decoction of Shenshuai Recipe. The removal rate of K+ was 62.5%-80.3% in other decoction of renal disease prescription. This technology was greatly reduced the intake of K+through decoction in patients with renal failure. In addition, it was found that the adsorption effect of this medicinal resin on K+ was greatly affected by the ion environment, and this kind of resin was also selective for the adsorption of different indicative component. CONCLUSION The establishment of this process of K+ removal can well reduce K+ in the decoction of Shenshuai Recipe. |
| Key words: Shenshuai Recipe potassium ion cation exchange resin sodium polystyrene sulfonate static adsorption |
