| 引用本文: | 嵇书霞,陈奕灵,钱跹,须秋萍,赵亮,李盛建.钩藤代煎液中6种生物碱的稳定性研究[J].中国现代应用药学,2023,40(24):3411-3417. |
| JI Shuxia,CHEN Yiling,QIAN Xian,XU Qiuping,ZHAO Liang,LI Chengjian.Study on the Stability of Six Alkaloids in Uncaria Decoction[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(24):3411-3417. |
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| 摘要: |
| 目的 建立超高效液相色谱串联质谱法（UHPLC-MS/MS）测定钩藤代煎液中钩藤碱、异钩藤碱、去氢钩藤碱、异去氢钩藤碱、毛钩藤碱和去氢毛钩藤碱的含量，考察不同储存条件对有效成分的放置稳定性的影响。方法 采用UHPLC-MS/MS，流动相为乙腈-0.1%甲酸水溶液，线性梯度洗脱，流速为0.4 mL·min–1，柱温为30℃；进样量为2 μL，质谱检测采用动态多反应离子检测模式；考察不同储存温度和储存时间对钩藤代煎液中6种有效成分稳定性的影响。结果 钩藤碱、异钩藤碱、去氢钩藤碱、异去氢钩藤碱、毛钩藤碱和去氢毛钩藤碱运用本法被成功分离，且在相应浓度范围内线性关系良好，其精密度、重复性、稳定性和加样回收率均良好；低温储存的药液14 d内6种生物碱基本稳定，常温储存3 d内基本稳定，高温储存较不稳定。结论 所建立的UHPLC-MS/MS稳定、快速、重复性好，可用于测定钩藤代煎液中6种有效成分的含量；冷藏有利于钩藤代煎液的稳定性。 |
| 关键词: 钩藤代煎液 超高效液相色谱串联质谱 稳定性 |
| DOI：10.13748/j.cnki.issn1007-7693.20222012 |
| 分类号:R284.1 |
| 基金项目:上海市宝山区医学特色专科和社区项目建设计划项目（BSZK-2019-C03）；上海市宝山区科技创新专项资金项目（19-E-55）；上海市宝山区卫生健康委优青（育才）计划（BSWSYC-2023-14） |
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| Study on the Stability of Six Alkaloids in Uncaria Decoction |
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JI Shuxia1, CHEN Yiling2, QIAN Xian1, XU Qiuping1, ZHAO Liang1, LI Chengjian1
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1.Shanghai Baoshan Luodian Hospital, Shanghai 201908, China;2.Shanghai Health Medical College, Shanghai 201308, China
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| Abstract: |
| OBJECTIVE To develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for determination of hynchophylline, isorhynchophylline, isocorynoxeine, corynoxeine, hirsutine and hirsuteine in Uncaria decoction, and investigate the effect of different storage conditions on the stability of active ingredients. METHODS An UHPLC-MS/MS method was established to investigate the effects of different storage temperature and time on the stability of six active components in Uncaria decoction. Separation was performed with a gradient mobile phase system of acetonitrile-0.1% formic water solution, the flow rate was 0.4 mL·min-1, the temperature of column was 30℃, the injection volume was 2 μL, the MS detection was dynamic multiple reaction monitoring mode. The effects of different storage temperature and time on the stability of six active components in Uncaria decoction was investigated. RESULTS Rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine, hirsutine and hirsuteine were successfully separated using this method, with good linear relationship in the corresponding concentration range. The precision, repeatability, stability and recovery rate were good. The six alkaloids were basically stable within 14 d under low temperature storage, basically stable within 3 d under normal temperature storage, and not stable under high temperature storage. CONCLUSION The UHPLC-MS/MS method is stable, rapid and reproducible. It can be used to determine the contents of six effective components in Uncaria decoction. Cold storage is beneficial to the stability of Uncaria decoction. |
| Key words: Uncaria decoction UHPLC-MS/MS stability |
