| 引用本文: | 洪亮,王福云,潘映秋,卢敏.复可托产品及原料中6种猪病毒基因膜芯片检测方法研究[J].中国现代应用药学,2022,39(23):3110-3116. |
| HONG Liang,WANG Fuyun,PAN Yingqiu,LU Min.Study on Detection Method of Six Porcine Viruses in Fucotto Products and Raw Materials by Gene Membrane Chip[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(23):3110-3116. |
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| 摘要: |
| 目的 利用基因膜芯片技术建立复可托产品及原料中6种猪病毒快速检测方法。方法 设计6种猪病毒特异性引物及探针，对样本核酸进行多重PCR扩增，进一步利用基因膜芯片技术进行产物杂交并分析判定。结果 建立的检测方法特异性强，稳定性好，灵敏度高，综合检测限为1.0×105 copies·mL-1，满足日常检验要求。结论 建立了6种猪病毒PCR-基因膜芯片技术的快速筛查方法，可保障复可托产品及原料的质量安全，该技术可进一步应用于养殖场和市场的猪肉检测，为政府监管部门提供有力的技术支撑。 |
| 关键词: 复可托 猪病毒 基因膜芯片 多重PCR |
| DOI：10.13748/j.cnki.issn1007-7693.2022.23.009 |
| 分类号:R917 |
| 基金项目:台州市科技计划项目(20ywb109) |
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| Study on Detection Method of Six Porcine Viruses in Fucotto Products and Raw Materials by Gene Membrane Chip |
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HONG Liang1, WANG Fuyun2, PAN Yingqiu1, LU Min1
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1.Taizhou Institute for Drug Control, Taizhou 318000, China;2.Zhejiang Feng'an Biopharmaceutical Co., Ltd., Taizhou 318000, China
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| Abstract: |
| OBJECTIVE To establish a rapid detection method of six porcine viruses in fucotto products and raw materials by using gene membrane chip technology. METHODS Six specific primers and probes of porcine viruses were designed to amplify the sample nucleic acid by PCR, and the products were hybridized and analyzed by gene membrane chip technology. RESULTS The established detection method had strong specificity, good stability and high sensitivity. The comprehensive detection limit was 1.0×105 copies·mL-1, which meet the requirements of daily inspection. CONCLUSION The rapid screening method of PCR gene membrane chip technology for six porcine viruses has been established, which can ensure the quality and safety of fucotto products and raw materials. This technology can be further applied to the detection of pork in farms and markets, and provide strong technical support for government regulators. |
| Key words: fucotto porcine virus gene membrane chip multiplex PCR |
