基于简易雾化设备脂多糖吸入诱导小鼠急性肺炎模型研究

业康; 李振皓; 蔡犇; 黄晶晶; 谢珍; 金捷; 金祖汉; 余琪; 王志安

引用本文:	业康,李振皓,蔡犇,黄晶晶,谢珍,金捷,金祖汉,余琪,王志安.基于简易雾化设备脂多糖吸入诱导小鼠急性肺炎模型研究[J].中国现代应用药学,2023,40(7):888-893.
	YE Kang,LI Zhenhao,CAI Ben,HUANG Jingjing,XIE Zhen,JIN Jie,JIN Zuhan,YU Qi,WANG Zhi'an.Study on Acute Pneumonia Induced by Inhalation of Lipopolysaccharide in Mice Based on Simple Atomizing Equipment[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(7):888-893.

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基于简易雾化设备脂多糖吸入诱导小鼠急性肺炎模型研究
业康¹, 李振皓², 蔡犇³, 黄晶晶¹, 谢珍⁴, 金捷¹, 金祖汉¹, 余琪¹, 王志安¹
1.浙江省中药研究所有限公司, 杭州 310023;2.浙江寿仙谷植物药研究院有限公司, 杭州 311121;3.浙江永宁药业股份有限公司, 浙江台州 318020;4.浙江省食品药品检验研究院, 杭州 310052

摘要:

目的评价简易雾化设备构建的脂多糖吸入诱导小鼠急性肺炎模型。方法脂多糖雾化吸入造模后,不同时间点观察小鼠体质量与肺灌洗液中总蛋白、一氧化氮(nitric oxide,NO)、白细胞水平的变化,同时对其肺组织进行HE、Masson以及TUNEL染色病理学检测,观察模型构建后动物的恢复情况。结果与对照组比较,造模后模型小鼠体质量显著下降(P<0.01),肺灌洗液中总蛋白水平与白细胞数显著上升(P<0.01),NO水平显著降低(P<0.01)。肺部病理学检查结果表明,模型组小鼠肺组织损伤严重,并出现纤维化,且存在大量细胞凋亡,与对照组有显著差异。结论利用简易雾化设备可成功构建脂多糖诱导的小鼠急性肺炎模型,模型较为稳定,可用于急性肺炎药效的筛选。

关键词: 脂多糖急性肺炎雾化吸入动物模型

DOI：10.13748/j.cnki.issn1007-7693.20220232

分类号:R965.1

基金项目:

Study on Acute Pneumonia Induced by Inhalation of Lipopolysaccharide in Mice Based on Simple Atomizing Equipment

YE Kang¹, LI Zhenhao², CAI Ben³, HUANG Jingjing¹, XIE Zhen⁴, JIN Jie¹, JIN Zuhan¹, YU Qi¹, WANG Zhi'an¹

1.Zhejiang Research Institute of Traditional Chinese Medicine Co., Ltd., Hangzhou 310023, China;2.Zhejiang Shouxiangu Botanical Drug Institute Co., Ltd., Hangzhou 311121, China;3.Zhejiang Yongning Pharmaceutical Co., Ltd., Taizhou 318020, China;4.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China

Abstract:

OBJECTIVE To evaluate the mouse model of acute pneumonia induced by lipopolysaccharide inhalation constructed by simple atomization equipment. METHODS After lipopolysaccharide atomization inhalation, the changes of body weight, total protein, nitric oxide(NO) and leukocyte levels in lung lavage fluid of mice were observed at different time points. At the same time, HE, Masson and TUNEL staining were performed on the lung tissue for pathological detection. Observed the recovery of animals after model construction. RESULTS Compared with the control group, the body weight of the model mice decreased significantly(P<0.01), the total protein level and the number of white blood cells in the lung lavage fluid significantly increased(P<0.01), and the NO level significantly decreased(P<0.01) after modeling. The results of lung pathological examination showed that the lung tissue of the model group was seriously damaged, fibrosis occurred, and a large number of cells apoptosis, which was significantly different from that of the control group. CONCLUSION The mouse model of acute pneumonia induced by lipopolysaccharides can be successfully constructed by using a simple atomization. The model is relatively stable and can be used to screen the drug efficacy.

Key words: lipopolysaccharides acute pneumonia atomization animal model