| 引用本文: | 时佩,鲁寅生,冯有龙,黄萍.离子色谱法测定小儿止咳糖浆中氯化铵的含量[J].中国现代应用药学,2022,39(12):1637-1639. |
| SHI Pei,LU Yinsheng,FENG Youlong,HUANG Ping.Determination of Ammonium Chloride in Xiao'er Zhike Syrup by Ion Chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(12):1637-1639. |
|
| 摘要: |
| 目的 建立离子色谱法测定小儿止咳糖浆中氯化铵含量的方法。方法 离子色谱法采用IonPacTM AS11-HC阴离子交换色谱柱(2.0 mm×250 mm)与IonPacTM AG11-HC保护柱(2.0 mm×50 mm)，检测器为抑制电导检测器，电导检测池温度为35℃，柱温为30℃，进样量为25 μL，以10 mmol·L–1氢氧化钾溶液为流动相，流速为0.5 mL·min–1。结果 氯离子浓度为10~400μg·mL–1时，峰面积与其浓度呈良好的线性关系(r=1.000 0，n=6);平均回收率为100.57%(RSD=0.41%，n=6)。结论 该方法专属性强、准确度高，可用于小儿止咳糖浆中氯化铵的含量测定。 |
| 关键词: 离子色谱法 小儿止咳糖浆 氯化铵 氯离子 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.12.019 |
| 分类号:R917.101 |
| 基金项目: |
|
| Determination of Ammonium Chloride in Xiao'er Zhike Syrup by Ion Chromatography |
|
SHI Pei1, LU Yinsheng2, FENG Youlong2, HUANG Ping1
|
|
1.College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;2.Jiangsu Institute for Food and Drug Control, Nanjing 210019, China
|
| Abstract: |
| OBJECTIVE To establish an ion chromatography method for determination of ammonium chloride in Xiao'er Zhike syrup. METHODS The chromatographic separation was performed on an IonPacTMAS11-HC anion exchange column (2.0 mm×250 mm) and IonPacTM AG11-HC guard column(2.0 mm×50 mm), using suppressed conductivity detector, the temperature of the detection tank and the column were set at 35℃ and 30℃ respectively, the injection volume was 25 μL, an isocratic eluent of 10 mmol·L-1 KOH was chosen as mobile phase and the flow rate was 0.5 mL·min-1. RESULTS A good linear relationship between the peak area and its concentration was observed within the range of 10-400 μg·mL-1(r=1.000 0, n=6); The average recovery was 100.57%(RSD=0.41%, n=6).CONCLUSION The method is specific and accurate, and can be used for determination of ammonium chloride in Xiao'er Zhike syrup. |
| Key words: ion chromatography Xiao'er Zhike syrup ammonium chloride chloride |
