| 引用本文: | 黄家欣,黄荣增,张丽君,宋成武,尹久,金姝娜.高脂血症与血清游离脂肪酸含量的相关性及辛伐他汀的干预作用[J].中国现代应用药学,2023,40(6):743-750. |
| HUANG Jiaxin,HUANG Rongzeng,ZHANG Lijun,SONG Chengwu,YIN Jiu,JIN Shuna.Correlation Between Hyperlipidemia and the Concentration of Free Fatty Acids in Serum and the Intervention Effect of Simvastatin[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(6):743-750. |
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| 高脂血症与血清游离脂肪酸含量的相关性及辛伐他汀的干预作用 |
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黄家欣1, 黄荣增1, 张丽君2, 宋成武1, 尹久3, 金姝娜2
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1.湖北中医药大学, 药学院, 武汉 430065;2.湖北中医药大学, 基础医学院, 武汉 430065;3.宜昌市东阳光实业发展有限公司, 湖北 宜昌 443300
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| 摘要: |
| 目的 利用气相色谱质谱联用技术(GC-MS)建立并验证血清中游离脂肪酸(free fatty acids，FFAs)的定量分析方法，研究血清FFAs含量与高脂血症之间的相关性及辛伐他汀对其的干预作用。方法 采用衍生化方法对血清样品进行甲酯化前处理；高脂饲料诱发高脂血症动物模型，辛伐他汀干预给药4周；利用GC-MS建立血清FFAs含量的分析方法并测定各组小鼠血清FFAs含量。结果 成功建立并验证GC-MS测量血清FFAs含量的分析方法，结果发现高脂血症小鼠血清中辛酸、十三烷酸、亚油酸等19个FFAs含量显著变化，且辛伐他汀对其中的14个FFAs具有显著的改善作用。结论 血清中FFAs含量与高脂血症密切相关，辛伐他汀对血清FFAs含量具有显著的改善作用，可为脂代谢相关疾病的早期检测和预后评估提供科学依据。 |
| 关键词: 游离脂肪酸 高脂血症 辛伐他汀 气相色谱-质谱联用 |
| DOI：10.13748/j.cnki.issn1007-7693.20213258 |
| 分类号:R917 |
| 基金项目:国家自然科学基金项目(81703243) |
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| Correlation Between Hyperlipidemia and the Concentration of Free Fatty Acids in Serum and the Intervention Effect of Simvastatin |
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HUANG Jiaxin1, HUANG Rongzeng1, ZHANG Lijun2, SONG Chengwu1, YIN Jiu3, JIN Shuna2
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1.Hubei University of Chinese Medicine, College of Pharmacy, Wuhan 430065, China;2.Hubei University of Chinese Medicine, College of Basic Medicine, Wuhan 430065, China;3.Yichang Dongyangguang Industrial Development Co., Ltd., Yichang 443300, China
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| Abstract: |
| OBJECTIVE To establish the present study of a quantitative analysis method to determine the concentration of free fatty acids(FFAs) in serum by gas chromatography-mass spectrometry(GC-MS). To investigate the correlation between the FFAs levels and hyperlipidemia in serum and the intervention effect of simvastatin. METHODS Derivatization was used to obtain methyl product. High-fat diet was used to induce hyperlipidemia animal model. The positive group was treated with simvastatin for four weeks. Meanwhile, the analysis method of serum FFAs was established by GC-MS to obtain the concentration of FFAs. RESULTS The GC-MS method was successfully established and validated for measuring the concentration of FFAs in serum. The results found that the nineteen FFAs were detected in serum of animals, such as caprylic acid, tridecylic acid, linoleic acid, etc. Simvastatin had a significant improvement effect on fourteen serum FFAs. CONCLUSION The content of FFAs in the serum is closely related to hyperlipidemia. Simvastatin can significantly ameliorate the distribution condition serum FFAs, which can provide a scientific basis for the early detection and prognosis evaluation of lipid metabolism-related diseases. |
| Key words: free fat acids hyperlipoidemia simvastatin GC-MS |
