| 引用本文: | 尤淞涛,徐莉莉,狄斌.氟标签衍生化联合氟固相萃取法在多肽纯化中的应用[J].中国现代应用药学,2021,38(1):108-114. |
| YOU Songtao,XU Lili,DI Bin.Application of Fluorinated Affinity Tags and Fluorous Solid-phase Extraction in Peptides Purification[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(1):108-114. |
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| 摘要: |
| 氟固相萃取（fluorous solid-phase extraction，FSPE）是一项基于氟-氟相互作用的固相萃取技术，它依靠高氟化目标化合物和高氟化固定相之间的亲和力，将目标化合物保留在固定相上，然后通过亲氟试剂将目标化合物洗脱下来，实现对目标化合物的分离和纯化。近年来，FSPE被广泛应用于多肽的富集和纯化。但由于多肽中一般不含有高氟化官能团，所以在多肽分子上连接此类高氟化的标签成为了FSPE应用于多肽纯化的关键。本文对氟亲和标签引入多肽的方式、种类以及其在多肽合成、纯化和富集中的应用和相关技术进行总结和阐述。 |
| 关键词: 氟固相萃取|氟标签|多肽纯化 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.01.018 |
| 分类号: |
| 基金项目:国家自然科学基金项目（81773693，81803354） |
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| Application of Fluorinated Affinity Tags and Fluorous Solid-phase Extraction in Peptides Purification |
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YOU Songtao, XU Lili, DI Bin
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Department of Pharmaceutical Analysis, Key Laboratory on Protein Chemistry and Structural Biology, China Pharmaceutical University, Nanjing 210009, China
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| Abstract: |
| Fluorous solid-phase extraction(FSPE) is a solid-phase extraction technology based on fluorine-fluorine interactions. Retaining on the perfluoroalkyl-functionalized stationary phase, and then eluted with a fluorophilic reagent, the perfluoroalkyl-containing target compound is separated and purified. In recent years, FSPE has been widely used in the enrichment and purification of peptides. However, because peptides normally do not contain highly fluorinated functional groups, attaching such highly fluorinated tags to peptide molecules has become the key to the application of FSPE to peptide analysis. This review summarized and elaborated the strategies and types of introduction of fluorinated affinity tags into peptides, their applications and related technologies in peptide synthesis, purification and enrichment. |
| Key words: fluorous solid-phase extraction(FSPE)|fluorinated affinity tags|peptides purification |
