| 引用本文: | 刘敏,张晓芹,黄绳武,余乐,范蕾,陈梦,陈张金,陈桂云,陈琴鸣,顾正兵.畲药树参茎化学成分分析及抗炎活性部位高效液相指纹图谱的建立[J].中国现代应用药学,2022,39(19):2505-2510. |
| LIU Min,ZHANG Xiaoqin,HUNG Shengwu,YU Le,FAN Lei,CHEN Meng,CHEN Zhangjin,CHEN Guiyun,CHEN Qinming,GU Zhengbing.Analysis Chemical Constituents in the Stems of She Medicine Dendropanax Dentiger and Establishing HPLC Fingerprint of Its Anti-inflammatory Active Fraction[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(19):2505-2510. |
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| 畲药树参茎化学成分分析及抗炎活性部位高效液相指纹图谱的建立 |
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刘敏1, 张晓芹2, 黄绳武3, 余乐1, 范蕾1, 陈梦1, 陈张金1, 陈桂云1, 陈琴鸣1, 顾正兵4
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1.丽水市质量检验检测研究院, 浙江 丽水 323000;2.丽水市中医院, 浙江 丽水 323000;3.浙江中医药大学, 杭州 310053;4.泰州丹鼎生物科技有限公司, 江苏 泰州 225400
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| 摘要: |
| 目的 对畲药树参茎所含化学成分进行确证分析，并建立抗炎活性部位的高效液相色谱指纹图谱。方法 采用液相色谱-质谱联用、核磁共振波谱仪等分析仪器分离鉴定树参茎中的化学成分；采用高效液相色谱法建立抗炎活性部位指纹图谱：色谱柱为Waters SunfireTM C18(4.6 mm×250 mm，5 μm)，流动相为乙腈-混合水溶液(含甲酸0.2%和四氢呋喃0.2%)，梯度洗脱。检测波长为256 nm，柱温30 ℃，体积流量为1.0 mL·mim–1，分析时间为80 min，进样量为5 μL。结果 从树参茎中共分离鉴定出10个化合物，分别是绿原酸(1)、3,4-O-二咖啡酰基奎宁酸(2)、3,5-O-二咖啡酰基奎宁酸(3)、4,5-O-二咖啡酰基奎宁酸(4)、saikolignanoside A(5)、芦丁(6)、山柰酚-3-O-芸香糖苷(7)、紫丁香苷(8)、松柏醇(9)、芥子醛葡萄糖苷(10)；12批样品抗炎活性部位指纹图谱相似度为0.901~0.992，均符合要求。结论 树参茎中主要含有酚酸类、黄酮类、苯丙素类化合物；建立的指纹图谱不仅能体现树参茎抗炎的化学物质基础，而且鉴别方法简便、重复性好，可为树参茎的品质评价及主要抗炎活性成分的确证提供科学依据。 |
| 关键词: 树参茎 抗炎 活性部位 高效液相色谱法 指纹图谱 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.19.011 |
| 分类号:R284.1 |
| 基金项目:浙江省药品监督管理局科技计划项目(2020003) |
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| Analysis Chemical Constituents in the Stems of She Medicine Dendropanax Dentiger and Establishing HPLC Fingerprint of Its Anti-inflammatory Active Fraction |
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LIU Min1, ZHANG Xiaoqin2, HUNG Shengwu3, YU Le1, FAN Lei1, CHEN Meng1, CHEN Zhangjin1, CHEN Guiyun1, CHEN Qinming1, GU Zhengbing4
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1.Lishui Institute for Quality Inspection and Testing, Lishui 323000, China;2.Lishui Hospital of Traditional Chinese Medicine, Lishui 323000, China;3.Zhejiang Chinese Medical University, Hangzhou 310053, China;4.Taizhou Danding Biotechnology Co., Ltd., Taizhou 225400, China
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| Abstract: |
| OBJECTIVE To confirm and analyze the chemical constituents in the stems of Dendropanax dentiger,and establish the HPLC fingerprint of its anti-inflammatory active fraction. METHODS Compounds were isolated and identified by LC-MS,NMR and other analysis instruments.HPLC with Waters SunfireTM C18 column (4.6 mm×250 mm,5 μm) was used.Mobile phase consisted acetonitrile-aqueous solution including 0.2% formic acid and 0.2% tetrahydrofuran with gradient elution.Detection wavelength was 256 nm.Column temperature was 30℃.Flow rate was 1.0 mL·mim–1 and analytic time was 80 min.Injection volume was 5 μL.RESULTS Ten compounds were isolated and identified as chlorogenic acid (1),3,4-O-caffeoylquinic acid methyl ester (2),3,5-O-caffeoylquinic acid methyl ester (3),4,5-O-caffeoylquinic acid methyl ester (4),saikolignanoside A (5),rutin (6),kaempferol-3-O-rutinoside (7),syringin (8),coniferol (9),sinapaldehyde glucoside (10).Under the same fingerprint chromatogram condition,the methodological results were satisfactory to 12 batches of the anti-inflammatory active fractions.And the similarity range was 0.901-0.992.CONCLUSION The stems of Dendropanax dentiger mainly contain phenolic acids,flavonoids and phenylpropanoids.The established fingerprint can not only reflect the chemical basis of anti-inflammatory of the She medicine,but the identification method also is simple and reproducible.It can provide a scientific basis for the quality evaluation and the confirmation of main anti-inflammatory active components of the stems of Dendropanax dentiger. |
| Key words: stems of Dendropanax dentiger anti-inflammatory active fraction HPLC fingerprint |
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