| 引用本文: | 张彦,简仕武,杨燕妮,夏俭华,杨黎燕,唐一梅,张寒,余雨婷.离子液体辅助超声波法提取猫眼草中黄酮的研究[J].中国现代应用药学,2022,39(23):3055-3061. |
| ZHANG Yan,JIAN Shiwu,YANG Yanni,XIA Jianhua,YANG Liyan,TANG Yimei,ZHANG Han,YU Yuting.Study on the Extraction of Flavonoids from Euphorbia Lunulata Bge by Ionic Liquid Assisted Ultrasonic Method[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(23):3055-3061. |
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| 离子液体辅助超声波法提取猫眼草中黄酮的研究 |
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张彦1,2, 简仕武3, 杨燕妮4, 夏俭华5, 杨黎燕1, 唐一梅1, 张寒1, 余雨婷1
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1.西安医学院药学院, 西安 710021;2.西安交通大学, 陕西省中医药管理局七药资源及抗肿瘤重点实验室, 西安 710065;3.白河县中医医院药剂科, 陕西 安康 725899;4.福建中医药大学, 福州 350108;5.上海中医药大学, 上海 201203
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| 摘要: |
| 目的 优化离子液体辅助超声波法提取猫眼草中的黄酮工艺，为进一步提取猫眼草中的黄酮类化合物提供依据。方法 以离子液体为提取溶剂，辅助超声波法提取猫眼草中的黄酮。依次改变离子液体的种类、浓度，提取溶剂总体积与药材质量比，超声时间，超声温度。以总黄酮提取率为指标，确定单因素最佳条件，在单因素试验结果的基础上结合响应曲面设计优化最佳提取条件，再用HPLC测定山柰酚和金丝桃苷的含量探究不同离子液体对猫眼草中黄酮提取的变化规律。结果 最佳提取条件：离子液体种类为1-丁基-3-甲基咪唑溴盐、离子液体与醇的体积比为1∶6、提取溶剂总体积与药材质量比为50∶1、温度为40℃、超声时间为1h。在最佳条件下提取率为(15.8±0.9)%，相比传统提取率5.14%，提取率明显增加。结论 用离子液体法提取猫眼草植物黄酮可行。黄酮提取率与离子液体母核碳链长短不成正相关性。 |
| 关键词: 离子液体 超声 猫眼草 黄酮 高效液相色谱 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.23.002 |
| 分类号:R284.2 |
| 基金项目:国家自然科学基金青年基金项目(81903579)；榆林市科技局产学研项目(CXY-2020-092)；西安市科技局农业技术研发项目(21NYYF0062) |
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| Study on the Extraction of Flavonoids from Euphorbia Lunulata Bge by Ionic Liquid Assisted Ultrasonic Method |
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ZHANG Yan1,2, JIAN Shiwu3, YANG Yanni4, XIA Jianhua5, YANG Liyan1, TANG Yimei1, ZHANG Han1, YU Yuting1
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1.College of Pharmacy, Xi'an Medical University, Xi'an 710021, China;2.Xi'an Jiaotong University, Seven Pharmaceutical Resources and Anti-tumor Medicine Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine, Xi'an 710065, China;3.Pharmacy Division, Baihe County Hospital of Traditional Chinese Medicine, Ankang 725899, China;4.Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;5.Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
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| Abstract: |
| OBJECTIVE To optimize the process of extracting flavonoids from Euphorbia lunulata Bge by ionic liquid assisted ultrasonic method, and provide a basis for further extraction of flavonoids in Euphorbia lunulata Bge. METHODS Using ionic liquid as extraction solvent, extract flavonoids from Euphorbia lunulata Bge assisted with ultrasound method. The species and concentration of ionic liquids, the ratio of total volume of extracted solvent and the mass of medicinal materials, ultrasonic time, ultrasonic temperature were changed in turn. Taking the flavonoid extraction rate as the index, the optimal conditions of the single factor were determined, optimize the optimal extraction conditions based on the single factor experimental results combined with the responsive surface design. The contents of kaempferol and hypericin were then determined by HPLC to investigate the changes of different ionic liquids on flavonoid extraction in Euphorbia lunulata Bge. RESULTS The optimum extraction conditions: the type of ionic liquid was 1-butyl-3-methylimidazole bromide, the volume ratio of ionic liquid to alcohol was 1∶6, the ratio of the total volume of the extracted solvent to the mass of the medicinal material was 50∶1, the temperature was 40 ℃, and the ultrasonic time was 1 h. The extraction rate was (15.8±0.9)% under the optimal conditions, which was significantly increased compared to the conventional extraction rate of 5.14%. CONCLUSION It is feasible to extract flavonoids from Euphorbia lunulata Bge by using ionic liquid as solvent. Flavonoid extraction rate is not positively correlated with the carbon chain length of the ionic liquid parent nucleus. |
| Key words: ionic liquid ultrasonography Euphorbia lunulata Bge flavonoids HPLC |
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