| 引用本文: | 陈良,徐晓琴,蒋兵.炮制对天山假狼毒毒性及化学成分的影响[J].中国现代应用药学,2021,38(24):3071-3077. |
| CHEN Liang,XU Xiaoqin,JIANG Bing.Effects of Processing on Toxicity and Chemical Components of Stelleropsis Tianschanica[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(24):3071-3077. |
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| 炮制对天山假狼毒毒性及化学成分的影响 |
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陈良1,2,3, 徐晓琴2,3,4, 蒋兵1
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1.新疆医科大学附属中医医院, 新疆中药炮制研究重点实验室, 乌鲁木齐 830000;2.中国科学院新疆理化技术研究所, 乌鲁木齐 830011;3.中国科学院大学, 北京 100049;4.新疆维吾尔自治区中药民族药研究所, 乌鲁木齐 830002
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| 摘要: |
| 目的 研究天山假狼毒炮制前后的急性毒性变化及其体内外组分的改变。方法 将小鼠分为生药组1（60.6 g·kg-1）、生药组2（75.75 g·kg-1）、生药组3（90.9 g·kg-1）、生药组4（106.05 g·kg-1）、生药组5（121.1 g·kg-1）、炮制组1（58.6 g·kg-1）、炮制组2（73.25 g·kg-1）、炮制组3（87.9 g·kg-1）、炮制组4（102.55 g·kg-1）、炮制组5（117.2 g·kg-1）（均按生药计），分别灌服不同浓度的天山假狼毒生药及炮制品提取物1次，连续观察14 d，记录小鼠急性毒性反应及动物死亡情况，计算LD50。利用UPLC-Q/TOF-MS测定天山假狼毒生药、炮制品及其含药血清总离子流图，分析各部组分的化学成分变化。结果 天山假狼毒生药提取物LD50=91.465 g·kg-1，95%置信区间为83.929~98.680 g·kg-1。天山假狼毒炮制品提取物LD50=104.900 g·kg-1，95%置信区间为95.584~122.774 g·kg-1（均按生药计）。死亡动物解剖仅见肝脏颜色变深，其他脏器肉眼未见明显改变。生药经炮制后7个成分峰面积减小，3个成分未出现。相较于炮制品，生药含药血清中的代谢成分或移行成分明显更多，且炮制品入血成分色谱峰明显小于生药。结论 炮制可明显降低天山假狼毒急性毒性，这种减毒作用与天山假狼毒部分成分含量的降低或消除有关。 |
| 关键词: 天山假狼毒 中药炮制 急性毒性试验 UPLC-Q/TOF-MS 化学成分 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.24.003 |
| 分类号:R285.5 |
| 基金项目:新疆医科大学附属中医医院院级课题（ZYY2019PZ03） |
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| Effects of Processing on Toxicity and Chemical Components of Stelleropsis Tianschanica |
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CHEN Liang1,2,3, XU Xiaoqin2,3,4, JIANG Bing1
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1.Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Processing and Research of Traditional Chinese Medicine, Urumqi 830000, China;2.Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.Xinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China
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| Abstract: |
| OBJECTIVE To study the changes of the acute toxicity and component in vitro and vivo of Stelleropsis tianschanica by pre and post processing. METHODS The mice were divided into crude drug group 1(60.6 g·kg-1), crude drug group 2(75.75 g·kg-1), crude drug group 3(90.9 g·kg-1), crude drug group 4(106.05 g·kg-1), crude drug group 5(121.1 g·kg-1), processing group 1(58.6 g·kg-1), processing group 2(73.25 g·kg-1), processing group 3(87.9 g·kg-1), processing group 4(102.55 g·kg-1), processing group 5(117.2 g·kg-1)(all calculated by crude drugs). Stelleropsis tianschanica crude drug and processed product extracts of different concentrations were administrated once, and observed continuously for 14 d. Acute toxicity reaction in mice and animals' death were recorded, and LD50 was calculated. Analyzed the total ion current of Stelleropsis tianschanica crude drug, processed products and drug-containing serum based on UPLC-Q/TOF-MS, and compared the composition changes of each component. RESULTS The LD50 of Stelleropsis tianschanica crude drug extract was 91.465 g·kg-1, and the 95% confidence interval was 83.929-98.680 g·kg-1. The LD50 of Stelleropsis tianschanica processed product extract was 104.900 g·kg-1, and the 95% confidence interval was 95.584-122.774 g·kg-1(all based on crude drugs). Only a darkened liver was observed in the dissection of dead animals, while there was no obvious changes founded in other organs. After processing, the chromatographic peak area of the 7 components was reduced, and the 3 components do not appear. Compared with processed products, there were significantly more metabolic or migrating components in the medicated serum of crude drugs, and the chromatographic peak of the blood components of processed products was significantly smaller than that of crude drugs. CONCLUSION Processing can significantly reduce the acute toxicity of Stelleropsis tianschanica, and this effect is related to the reduction or elimination of some components of Stelleropsis tianschanica. |
| Key words: Stelleropsis tianschanica Chinese medicinal herbs processing acute toxicity test UPLC-Q/TOF-MS chemical composition |
