| 引用本文: | 徐清妍,陈琪,钟瑞生,朱晓燕,郑玛丽.十六烷基乙醇胺对睡眠剥夺诱发小鼠干眼症的影响[J].中国现代应用药学,2022,39(12):1620-1626. |
| XU Qingyan,CHEN Qi,ZHONG Ruisheng,ZHU Xiaoyan,ZHENG Mali.Effect of Palmitoylethanolamide on Sleep Deprivation-induced Dry Eye in Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(12):1620-1626. |
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| 摘要: |
| 目的 研究内源性促炎症消退脂质十六烷基乙醇胺对睡眠剥夺诱发小鼠干眼症的影响。方法 以“水上站立”方式建立睡眠剥夺小鼠模型，每天剥夺小鼠睡眠时间为20 h，检测5 d和10 d造模组及正常组小鼠泪腺组织中PPAR-α基因及蛋白表达，检测十六烷基乙醇胺含量及其合成酶NAPEPLD、代谢酶NAAA基因表达；分别给予十六烷基乙醇胺及其溶剂，检测各组小鼠泪液分泌量、角膜损伤及结膜杯状细胞表达，评价泪腺组织病理学变化。结果 与正常组比较，模型组小鼠泪腺组织中十六烷基乙醇胺及其受体PPAR-α表达明显减低，合成酶NAPEPLD表达下降，而降解酶NAAA基因水平表达升高。与空白溶剂治疗组相比较，十六烷基乙醇胺能增加睡眠剥夺小鼠泪液分泌量，减少角膜损伤，显著增加结膜杯状细胞数量，改善模型小鼠泪腺组织脂质沉积及病理学变化，维持泪腺基本骨架。结论 十六烷基乙醇胺能够改善睡眠剥夺诱导的小鼠干眼症。 |
| 关键词: 十六烷基乙醇胺 睡眠剥夺 干眼症 泪腺 脂质沉积 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.12.017 |
| 分类号:R965.1 |
| 基金项目:福建中医药大学校管课题临床专项项目(XB2020051) |
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| Effect of Palmitoylethanolamide on Sleep Deprivation-induced Dry Eye in Mice |
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XU Qingyan1, CHEN Qi2, ZHONG Ruisheng1, ZHU Xiaoyan1, ZHENG Mali1
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1.Department of Ophthalmology, Xiamen TCM Hospital, Xiamen 361009, China;2.Eye Institute of Xiamen University, Xiamen 361102, China
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| Abstract: |
| OBJECTIVE To study the effect of endogenous pro-inflammatory lipid palmitoylethanolamide on sleep deprivation-induced dry eye in mice. METHODS Sleep deprivation-induced mouse model was established by "stick over water" and last for 20 h every day. PPAR-α gene and protein expression were detected in lacrimal gland tissues of 5, 10 d model group and normal group. The expression of palmitoylethanolamide and the related synthetic enzyme NAPEPLD and metabolizing enzyme NAAA were also detected; cetylethanolamine and its solvent were administered respectively, the lacrimal gland, tear secretion, corneal injury and expression of conjunctival goblet cells were detected, histopathological changes of lacrimal gland were evaluated. RESULTS Compared with the normal group, the expression of cetylethanolamine and its receptor PPAR-α in the lacrimal gland tissue of the mice in the model group was significantly decreased, the expression of the synthase NAPEPLD was decreased, and the gene expression of the degrading enzyme NAAA was increased. Compared with the blank solvent treatment group, cetylethanolamine could increase the secretion of tears in sleep-deprived mice, reduce corneal damage, and significantly increase the number of conjunctival goblet cells, improve the lipid deposition and pathological changes in the lacrimal gland tissue of model mice, and maintain the basic skeleton of the lacrimal gland.CONCLUSION Palmitoylethanolamide can improve sleep deprivation induced dry eye in mice. |
| Key words: palmitoylethanolamide sleep deprivation dry eye lacrimal gland lipid accumulation |
