| 引用本文: | 肖观林,钟惠娴,黄华靖,曾志浩,毕晓黎.UPLC-Q-TOF-MS/MS鉴定小儿清咽颗粒化学成分[J].中国现代应用药学,2022,39(12):1627-1636. |
| XIAO Guanlin,ZHONG Huixian,HUANG Huajing,ZENG Zhihao,BI Xiaoli.Identification of Chemical Constituents in Xiao'er Qingyan Granules by UPLC-Q-TOF-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(12):1627-1636. |
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| UPLC-Q-TOF-MS/MS鉴定小儿清咽颗粒化学成分 |
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肖观林1,2, 钟惠娴3, 黄华靖3, 曾志浩3, 毕晓黎1,2
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1.广东省中医药工程技术研究院, 广州 510095;2.广东省中医药研究开发重点实验室, 广州 510095;3.广州中医药大学第五临床医学院, 广州 510405
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| 摘要: |
| 目的 基于超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS/MS)技术鉴定小儿清咽颗粒中的化学成分。方法 采用Phenomenex Kinetex XB C18色谱柱(2.1 mm×150 mm，1.7 μm)，以0.1%甲酸水-乙腈为流动相体系，梯度洗脱，流速0.25 mL·min–1，质谱采用电喷雾(ESI)离子源，以正、负离子模式采集多级质谱碎片信息。结果 通过高分辨质谱数据分析，结合参考文献数据以及对照品确认，共鉴别出59个化学成分，包括5个环烯醚萜类，15个有机酸类，5个色原酮类，14个黄酮类，4个木脂素类，9个苯乙醇苷类，2个核苷类，2个酚类及3个其他类，并对化合物的药材来源进行了归属。结论 本研究建立的分析方法灵敏、高效，可应用于小儿清咽颗粒各类化学成分的鉴定分析，为研究小儿清咽颗粒药效物质基础和作用机制提供重要依据。 |
| 关键词: 小儿清咽颗粒 超高效液相色谱-四极杆-飞行时间串联质谱法 化学成分 裂解规律 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.12.018 |
| 分类号:R284.1 |
| 基金项目:广东省科技创新战略专项(部省市联动专项与省属科研机构稳定性支持)项目(粤财科教[2021]113号) |
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| Identification of Chemical Constituents in Xiao'er Qingyan Granules by UPLC-Q-TOF-MS/MS |
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XIAO Guanlin1,2, ZHONG Huixian3, HUANG Huajing3, ZENG Zhihao3, BI Xiaoli1,2
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1.Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine, Guangzhou 510095, China;2.Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou 510095, China;3.School of the Fifth Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
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| Abstract: |
| OBJECTIVE To identify the chemical constituents of Xiao'er Qingyan granules by using UPLC-Q-TOF-MS technology.METHODS The separation was performed on a Phenomenex Kinetex XB C18 column(2.1 mm×150 mm, 1.7 μm) with mobile phase of water(containing 0.1% formic acid)-acetonitrile with gradient elution at a flow rate of 0.25 mL·min-1. Electrospray ion(ESI) source was applied to collect multi-level mass spectrometry fragment information under positive and negative ion modes. RESULTS A total of 59 chemical constituents were identified by high mass spectrometry data analysis, literature comparison and reference confirmation. The chemical constituent cluster was composed of 5 iridoids, 15 organic acids, 5 chromones, 14 flavonoids, 4 lignans, 9 phenylethanoid glycosides, 2 nucleosides, 2 phenols and 3 other compounds. Furthermore, all of the constituents were surveyed and classified according to their medicinal materials derivation. CONCLUSION The proposed method is sensitive and efficient, and can be applied in identification analysis of various chemical constituents of Xiao'er Qingyan granules, which provides a crucial basis for further studying the material of Xiao'er Qingyan granules' efficacy and mechanism. |
| Key words: Xiao'er Qingyan granules UPLC-Q-TOF-MS/MS chemical constituents fragmentation pathway |
