| 引用本文: | 刘小伟,宝鲁尔,荣君,新图雅,陈红梅,王秀兰.一测多评法同时测定蒙药吉如合-6中6种成分含量[J].中国现代应用药学,2022,39(19):2457-2464. |
| LIU Xiaowei,BAO Lu'er,RONG Jun,XIN Tuya,CHEN Hongmei,WANG Xiulan.Simultaneous Determination of Six Components in Mongolian Medicine Jiruhe-6 by Quantitative Analysis of Multi-components by Single-marker[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(19):2457-2464. |
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| 一测多评法同时测定蒙药吉如合-6中6种成分含量 |
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刘小伟1, 宝鲁尔2, 荣君1, 新图雅2, 陈红梅1, 王秀兰2,3
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1.内蒙古民族大学附属医院, 内蒙古 通辽 028000;2.内蒙古民族大学蒙医药学院, 内蒙古 通辽 028000;3.内蒙古蒙医药工程技术研究院, 内蒙古 通辽 028000
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| 摘要: |
| 目的 利用一测多评法同时测定蒙药吉如合-6中鞣花酸、肉桂酸、丁香酚、木香烃内酯、去氢木香内酯、去氢二异丁香酚等6种成分的含量，并验证该方法在蒙药吉如合-6质量控制研究中的可行性与科学性。方法 采用一测多评法；供试品采用75%甲醇回流提取；以丁香酚为内标物，测定其他5种成分相对校正因子(ƒk/m)；采用一测多评法与外标法进行含量计算、比对。结果 所测定的鞣花酸、肉桂酸、丁香酚、木香烃内酯、去氢木香内酯、去氢二异丁香酚等6种成分分别在其各自范围内有良好的线性关系(即r>0.999 9)，平均加样回收率为94.80%~98.85%，RSD<2.0%，外标法与一测多评法所测得结果之间相对误差<2%。结论 一测多评法操作简便、准确可行、重复性好，可用于蒙药吉如合-6的质量控制。 |
| 关键词: 蒙药 吉如合-6 成分 一测多评法 外标法 校正因子 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.19.005 |
| 分类号:R284.2 |
| 基金项目:国家重点研发计划项目(2018YFC1708205);内蒙古自治区科技重大专项(2019ZD004);内蒙古自治区人民政府蒙医药标准化项目(2020-MB019) |
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| Simultaneous Determination of Six Components in Mongolian Medicine Jiruhe-6 by Quantitative Analysis of Multi-components by Single-marker |
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LIU Xiaowei1, BAO Lu'er2, RONG Jun1, XIN Tuya2, CHEN Hongmei1, WANG Xiulan2,3
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1.Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao 028000, China;2.School of Mongolia Medicine and Pharmacy, Inner Mongolia University for Nationalities, Tongliao 028000, China;3.Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology, Tongliao 028000, China
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| Abstract: |
| OBJECTIVE To determine the ellagic acid,cinnamic acid,eugenol,costunolide,dehydrocostus lactone and dehydrodiisoeugenol in Mongolian medicine Jiruhe-6 by quantitative analysis multi-components by single marker (QAMS).And to verify the feasibility and scientificity of this method in the quality control study of Mongolian medicine Jiruhe-6.METHODS QAMS method was used.The test sample was extracted with 75% methanol under reflux.Relative calibration factors (ƒk/m) of other 5 components were determined with eugenol as internal standard substance.QAMS and external standard method were used for content calculation and comparison.RESULTS There was a good linear relationship between ellagic acid,cinnamic acid,eugenol,costunolide,dehydrocostus lactone and dehydrodiisoeugenol in their respective ranges (r>0.999 9).The average recovery was 94.80%-98.85%,RSD<2.0%.The relative error between external standard method and QAMS was<2%.CONCLUSION QAMS method is simple,accurate,feasible and reproducible.It can be used for the quality standard control of Mongolian medicine Jiruhe-6. |
| Key words: Mongolian medicine Jiruhe-6 component quantitative analysis multi-components by single marker (QAMS) external standard method correction factor |
