| 引用本文: | 张明哲,戴明珠,梁云飞,郝二伟,侯小涛,邓家刚,叶祖光,吕林艳,刘洋,杜正彩.中华跌打丸调节钙通道及ATP代谢改善外周疲劳作用研究[J].中国现代应用药学,2022,39(24):3234-3239. |
| ZHANG Mingzhe,DAI Mingzhu,LIANG Yunfei,HAO Erwei,HOU Xiaotao,DENG Jiagang,YE Zuguang,LYU Linyan,LIU Yang,DU Zhengcai.Study on Anti-peripheral Fatigue Effect of Zhonghua Dieda Pills by Regulating Calcium Channel and ATP Metabolism[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(24):3234-3239. |
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| 中华跌打丸调节钙通道及ATP代谢改善外周疲劳作用研究 |
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张明哲1, 戴明珠2, 梁云飞3, 郝二伟4, 侯小涛4, 邓家刚4, 叶祖光5, 吕林艳3, 刘洋3, 杜正彩1
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1.广西中医药大学, 广西中医药科学实验中心, 南宁 530200;2.杭州环特生物科技股份有限公司, 杭州 310051;3.广西梧州制药(集团)股份有限公司, 广西 梧州 543000;4.广西中医药大学, 广西中药药效研究重点实验室, 南宁 530200;5.中国中医科学院中药研究所, 北京 100000
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| 摘要: |
| 目的 建立缺氧-疲劳斑马鱼模型,研究中华跌打丸抗外周疲劳作用及其机制。方法 利用亚硫酸钠诱导建立缺氧-疲劳模型,分别从运动能力、乳酸含量、ATP生成、镇痛、自由基清除和钙离子代谢6个方面考察中华跌打丸的抗疲劳作用。结果 中华跌打丸(250,500,1 000和2 000 µg·mL-1)可明显改善疲劳斑马鱼的运动能力,促进乳酸代谢,降低机体乳酸含量,同时具有镇痛作用;中华跌打丸1 000 µg·mL-1可明显促进疲劳斑马鱼ATP能量合成;中华跌打丸500,1 000和2 000 µg·mL-1可明显提高超氧化物歧化酶1(SOD1)基因相对表达量;中华跌打丸1 000,2 000 µg·mL-1可明显提高钙离子通道(CACNAIDA)基因相对表达量。结论 中华跌打丸具有明显的抗外周疲劳和镇痛作用,其机制可能与促进乳酸代谢、促进ATP生成、提高SOD1和CACNA1DA基因相对表达量有关。 |
| 关键词: 斑马鱼 中华跌打丸 抗疲劳 乳酸 ATP 超氧化物歧化酶1 钙离子通道 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.24.007 |
| 分类号:B285.5 |
| 基金项目:广西科技重大专项(桂科AA17202040-2)；广西中药药效研究重点实验室项目(17-259-20) |
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| Study on Anti-peripheral Fatigue Effect of Zhonghua Dieda Pills by Regulating Calcium Channel and ATP Metabolism |
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ZHANG Mingzhe1, DAI Mingzhu2, LIANG Yunfei3, HAO Erwei4, HOU Xiaotao4, DENG Jiagang4, YE Zuguang5, LYU Linyan3, LIU Yang3, DU Zhengcai1
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1.Guangxi University of Traditional Chinese Medicine, Guangxi Scientific Experimental Center of Traditional Chinese Medicine,, Nanning 530200, China;2.Hunter Biotechnology Group Co., Ltd., Hangzhou 310051, China;3.Guangxi Wuzhou Pharmaceutical Group Co., Ltd., Wuzhou 543000, China;4.Guangxi University of Traditional Chinese Medicine, Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Nanning 530200, China;5.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100000, China
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| Abstract: |
| OBJECTIVE To establish the zebrafish hypoxia-fatigue model, and to study the anti-peripheral fatigue effect of Zhonghua Dieda pills and its mechanism. METHODS The hypoxia-fatigue model of zebrafish was established by sodium sulfite. The anti-fatigue effects of Zhonghua Dieda pills were observed from six aspects:exercise, content of lactic acid, ATP production, degree of pain, free radical scavenging and calcium ion metabolism. RESULTS Zhonghua Dieda pills(250, 500, 1 000 and 2 000 µg·mL-1) could significantly improve the exercise ability of fatigued zebrafish, promote lactic acid metabolism, and reduce the content of lactic acid. Zhonghua Dieda pills 1 000 µg·mL-1 could significantly promote ATP energy synthesis of fatigued zebrafish, while having an analgesic effect. Zhonghua Dieda pills 500, 1 000 and 2 000 µg·mL-1 could significantly increase the relative expression of SOD1 gene. Zhonghua Dieda pills 1 000, 2 000 µg·mL-1 could significantly increase the relative expression of CACNA1DA gene. CONCLUSION Zhonghua Dieda pills has obvious anti-peripheral fatigue and analgesic effect, the mechanism maybe related to promoting lactate metabolism, promoting ATP production, increasing the relative expression of SOD1 gene and increasing the relative expression of CACNA1DA gene. |
| Key words: zebrafish Zhonghua Dieda pills anti-fatigue lactic acid ATP SOD1 CACNA1DA |
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