| 引用本文: | 王启帅,肖功胜,杨云,郝鹏飞.北柴胡气相指纹图谱数据的化学计量学分析[J].中国现代应用药学,2010,27(12):1097-1102. |
| .Chemometric Analysis on the GC Fingerprint Data of the Root of Bupleurum chinense DC[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(12):1097-1102. |
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| 摘要: |
| 目的 对北柴胡挥发性成分的气相指纹图谱进行化学计量学研究，提取出指纹图谱中的特征数据信息。方法 运用Agilent 7890A气相色谱仪，安捷伦HP-5毛细管气相色谱柱(30 m×0.25 mm，0.25 µm)，载气为氮气，流速为1.03 mL·min-1，FID检测温度为280 ℃，对不同产地北柴胡样品进行分析。运用指纹图谱处理软件对指纹图谱数据进行相似度分析、聚类分析及主成分分析。结果 建立了北柴胡挥发性成分的指纹图谱共有模式，获得了28个共有峰。依据化学计量学分析，将样品分成了3类。结论 结果表明化学计量学分析方法适用于指纹图谱数据的分析，可为定量研究提供依据，有助于柴胡药材的质量评价研究。 |
| 关键词: 北柴胡 气相指纹图谱 化学计量学 主成分分析 |
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| Chemometric Analysis on the GC Fingerprint Data of the Root of Bupleurum chinense DC |
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WANG Qishuai XIAO Gongsheng YANG Yun* HAO Pengfei
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| Abstract: |
| OBJECTIVE To study the gas chromatographic fingerprint of the volatile constituents from the root of Bupleurum chinense DC. and obtain the characteristic data based on chemometric analysis. METHODS Samples of B.chinense DC. from different habitats were determined by Aglient 7890A GC system with HP-5 capillary column (30 m×0.25 mm, 0.25 µm). Nitrogen was used as carrier gas and flow rate was 1.03 mL·min-1, FID temperature was 280 ℃. The methods of similarity, cluster analysis and principal component analysis were used to process the data from which was obtained gas chromatographic fingerprint. The assisted computational tool was “Chromafinger” solution software. RESULTS The common pattern of GC fingerprint was established. Twenty-eight co-possessing peaks were selected as the fingerprint peaks of B.chinense DC.. Samples were divided into three categories based on the analysis of chemometrics. CONCLUSION The results show that chemometrics is suitable for the analysis of fingerprint data, which can provide a basis for quantitative research. Chemometric analysis contributes to research the quality evaluation for B. chinense DC.. |
| Key words: Bupleurum chinense DC. GC fingerprint chemometrics principal component analysis |
