| 引用本文: | 黄茸茸1,李卫平2*,李维祖2.黄芪提取物对大鼠局灶性脑缺血再灌注血脑屏障损伤的影响[J].中国现代应用药学,2010,27(12):1064-1068. |
| .Effect of Extract of Astragalus on Alterations of Blood Brain Barrier Induced by Local Cerebral Ischemia-reperfusion Injury[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(12):1064-1068. |
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| 摘要: |
| 目的 研究黄芪提取物(extract of astragalus，EA)对大鼠局灶性脑缺血(MCAO)再灌注的血脑屏障损伤的影响。方法 采用线栓法制备MCAO再灌注模型，观察EA对缺血再灌注大鼠的神经功能障碍、脑梗死体积和脑含水量、外周血循环内皮细胞(CEC)含量、缺血脑组织中细胞间粘附分子(ICAM-1)的表达、缺血侧脑组织皮质血脑屏障超微结构的病理学变化的影响。结果 EA80 mg·kg-1，EA(40，80 mg·kg-1)、EA(20，40，80 mg·kg-1)可分别改善缺血再灌注2，8，24 h大鼠的神经功能障碍；能明显降低外周血中的CEC含量，明显减轻缺血脑组织的脑水肿和脑梗死体积，对脑缺血再灌注后血脑屏障超微结构的病理改变有一定的改善作用；能减少ICAM-1免疫反应阳性血管数。结论 EA对局灶性脑缺血再灌注损伤大鼠有一定的保护作用，作用机制可能与其保护血管内皮细胞，减轻脑缺血再灌注后的血脑屏障损伤有关。 |
| 关键词: 黄芪提取物 局脑缺血 再灌注损伤 血脑屏障 ICAM-1 血循环内皮细胞 |
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| Effect of Extract of Astragalus on Alterations of Blood Brain Barrier Induced by Local Cerebral Ischemia-reperfusion Injury |
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HUANG Rongrong1 LI Weiping2* LI Weizu2
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| Abstract: |
| OBJECTIVE To study the effect of extract of astragalus (EA) on alterations of blood brain barrier after local cerebral ischemia-reperfusion injury in rats. METHODS Middle cerebral artery occlusion (MCAO) was used to make local cerebral ischemia-reperfusion model with intravascular nylon filament occlusion. The neurological function score, brain edema and infarct volume, the counts of peripheral blood circulating cells, the expression of intercellular adhesion molecule-1, pathological changes in blood-brain barrier ultrastructure in rats after focal cerebral ischemia-reperfusion were observed. RESULTS EA 80 mg·kg-1 decreased the neurological function score; EA(40 and 80 mg·kg-1) reduced brain edema and infarct volume, the counts of peripheral blood circulating cells, pathological changes in blood-brain barrier ultrastructure; EA(20, 40 and 80 mg·kg-1) decreased the expression of intercellular adhesion molecule-1 in brain tissue. CONCLUSION EA has protective effects against cerebral ischemia reperfusion injuries, the mechanism may be related with reducing BBB injury induced by local cerebral ischemia-reperfusion. |
| Key words: extract of astragalus local cerebral ischemia reperfusion injury blood-brain barrier intercellular adhesion molecule-1 peripheral blood circulating cells |
