| 引用本文: | 梁健健,张蜀,邓红,林华庆,杨光耀.加替沙星聚乳酸纳米粒的包封率测定[J].中国现代应用药学,2010,27(11):1018-1020. |
| .Determination of Encapsulation Rate of Gatifloxacin-loaded Polylacticacid Nanoparticles[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(11):1018-1020. |
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| 摘要: |
| 目的 建立加替沙星聚乳酸纳米粒的包封率测定方法。方法 采用葡聚糖凝胶柱层析法，以水为洗脱剂，以l mL·min-1的流速洗脱，采用反相高效液相色谱法测定药物含量，计算包封率。色谱柱为C18柱(150 mm×4.6 mm，5 μm)，流动相为0.02 mol·L-1枸橼酸溶液(内含0.6%三乙胺)-甲醇(60∶40)，检测波长为290 nm，流速为1.0 mL·min-1，进样量20 μL。结果 加替沙星在2.051~60.14 μg·mL-1内线性关系良好(r=0.999 9)。平均柱回收率为98.8%，RSD=0.71%；测得3批纳米粒的平均包封率为82.1%，RSD为2.2%。结论 建立的方法可用于加替沙星聚乳酸纳米粒的包封率测定。 |
| 关键词: 加替沙星 聚乳酸纳米粒 包封率 葡聚糖凝胶柱法 |
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| Determination of Encapsulation Rate of Gatifloxacin-loaded Polylacticacid Nanoparticles |
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LIANG Jianjian1 ZHANG Shu2* DENG Hong2 LIN Huaqing2 YANG Guangyao2
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| Abstract: |
| OBJECTIVE To establish a method for the detection of encapsulation rate of gatifloxacin in gatifloxacin-polylacticacid nanoparticles. METHODS The analysis was performed on a sephadex G-50 column, using distilled water as eluent, and detected by HPLC with a C18 column (150 mm×4.6 mm, 5 μm) using 0.02 mol·L-1 citric acid (0.6% triethylamine)-methanol (60∶40) as mobile phase under the flow rate of 1.0 mL·min-1. The detection wavelength was 290 nm. RESULTS Gatifloxacin had a good linear range of 2.051-60.14 μg·mL-1(r=0.999 9). The average column recovery rate was 98.8% with RSD of 0.71%. The average encapsulation rate of 3 batchs nanoparticles was 82.1% with RSD of 2.2%. CONCLUSION The method can be used to detect the encapsulation rate of gatifloxacin in the nanoparticles. |
| Key words: gatifloxacin polylacticacid nanoparticles encapsulation rate sephadex G-50 |
