| 引用本文: | 高金波,孙丽娜,滕杨,侯巍.蒲公英根多糖的分离、纯化及结构的初步分析[J].中国现代应用药学,2010,27(10):905-908. |
| .Isolation, Purification and Analysis of Glycoprotein from Taraxacum Polysaccharides[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(10):905-908. |
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| 摘要: |
| 目的 提取蒲公英根多糖，并对该多糖进行分离、纯化和结构的初步分析。方法 采用超声波协同酶法进行提取多糖，分离纯化后，经Sephadex G-75柱分成A，B，C，D4级，采用优化的PMP柱前衍生HPLC分析单糖组成。采用紫外、红外等方法进行结构的初步分析。结果 4种多糖均含有D-鼠李糖、葡萄糖、D-半乳糖、D-木糖和D-阿拉伯糖；它们的单糖的摩尔比依次为，A：0.098∶2.88∶0.125∶0.01∶0.33；B：0.113∶0.68∶0.13∶ 0.011∶0.19；C：0.139∶2.46∶0.36∶0.13∶0.086；D：0.028∶0.36∶0.031∶0.012∶0.94。结论 4种多糖在结构上有明显的不同。 |
| 关键词: 蒲公英 多糖 结构 |
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| Isolation, Purification and Analysis of Glycoprotein from Taraxacum Polysaccharides |
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GAO Jinbo SUN Lina TENG Yang HOU Wei*
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| Abstract: |
| OBJECTIVE To extract polysaccharides from the root of Taraxacum and then isolate, purify and analyze the structure of polysaccharides. METHODS Polysaccharides were extracted by ultrasonic and coordinated enzyme methods, after separating and purifying, four levels (A, B, C, D) were fractionated by Sephadex G-75 column and then monosaccharide constituents were analyzed by HPLC applied to PMP precolumn derivation. Preliminary analysis of structure was analyzed by UV, IR method. RESULTS All of the four kinds of monosaccharides contain D-rhamnose, glucose, D-galactose, D-xylose and D-arabinose. Their molar ratios are as follow in turns: Fraction A: 0.098∶2.88∶0.125∶0.017∶0.33；B: 0.113∶0.68∶0.13∶ 0.011∶0.19; C: 0.139∶2.46∶0.36∶0.13∶0.086; D: 0.028∶0.36∶0.031∶0.012∶0.94. CONCLUSION It showed that there were significant differences in four species of polysaccharides. |
| Key words: Taraxacum polysaccharide structure |
