| 引用本文: | 王春艳,杨建春,于庆坤,张琦,潘卫三.HPLC-ELSD测定复方参芪软胶囊中黄芪甲苷的含量[J].中国现代应用药学,2010,27(9):846-848. |
| .Content Determination of Astragaloside in Compound Shenqi Soft Capsule by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(9):846-848. |
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| 摘要: |
| 目的 建立以高效液相色谱-蒸发光散射检测法测定复方参芪软胶囊中黄芪甲苷含量的方法。方法 色谱柱为Agilent Hypersil ODS，流动相为乙腈-水(35∶65)，流速为1.0 mL·min-1，进样量为20 μL；蒸发光散射检测器条件：载气(压缩空气)压力为0.32 MPa，载气流量：2.6 L·min-1，漂移管温度为108 ℃。结果 黄芪甲苷进样量的线性范围为0.402 4~4.024 0 µg (r=0.999 8)；平均回收率为100.02%(RSD=1.41%)。结论 该方法准确度高、专属性强，可用于该制剂中黄芪甲苷的含量测定。 |
| 关键词: 高效液相色谱-蒸发光散射检测法 复方参芪软胶囊 黄芪甲苷 含量测定 |
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| Content Determination of Astragaloside in Compound Shenqi Soft Capsule by HPLC-ELSD |
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WANG Chunyan1 YANG Jianchun1 YU Qingkun1 ZHANG Qi1 PAN Weisan2
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| Abstract: |
| OBJECTIVE To establish an HPLC-ELSD method for the determination of astragaloside in compound shenqi soft capsule. METHODS The samples were determined by HPLC-ELSD on an Agilent Hypersil ODS column using acetonitrile-water(35∶65) as the mobile phase at a flow rate of 1.0 mL·min-1. The injection volume was 20 μL. The ELSD conditions were as follows: pressure of compressed air was 0.32 MPa, the flow rate of compressed air was 2.6 L·min-1，drift temperature was 108 ℃. RESULTS The linear range of astragaloside was 0.402 4-4.024 0 µg (r=0.999 8) and the average recovery rate was 100.02%(RSD=1.41%). CONCLUSION The method is accurate, specific and applicable for the determination of astragaloside in compound shenqi soft capsule. |
| Key words: HPLC-ELSD compound shenqi soft capsule astragaloside content determination |
