| 引用本文: | 陈梅荣,王晖,袁海铭.HPLC测定不同产地枫树叶中没食子酸的含量[J].中国现代应用药学,2010,27(9):840-842. |
| .Determination of Gallic Acid in Leaf of Liquidambar taiwaniana Hance in Different Areas by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(9):840-842. |
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| 摘要: |
| 目的 建立高效液相色谱法测定不同产地枫树叶中没食子酸的含量。方法 色谱柱为Alltima C18(4.6 mm×250 mm，5 µm)；流动相为乙腈-0.05%磷酸溶液(4∶96)；流速为1.0 mL·min-1；检测波长为270 nm；柱温为30 ℃。结果 没食子酸进样浓度在0.019 08～0.229 0 mg·mL-1(r=0.999 6)内与峰面积呈良好的线性关系，平均回收率为101.52%，RSD为1.40%(n=6)。结论 该方法简便、准确、重复性好，可用于枫树叶的质量控制。 |
| 关键词: 高效液相色谱法 枫树叶 没食子酸 |
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| Determination of Gallic Acid in Leaf of Liquidambar taiwaniana Hance in Different Areas by HPLC |
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CHEN Meirong WANG Hui YUAN Haiming
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| Abstract: |
| OBJECTIVE To establish the method of determination of gallic acid in Leaf of Liquidambar taiwaniana Hance in different areas. METHODS The chromatographic conditions included Alltima C18 column(4.6 mm×250 mm, 5 μm) and the mobile phase consisted of acetonitril-0.05% phosphoric acid(4∶96). Detection wavelength was at 270 nm. The flow rate was 1.0 mL·min-1. Column temperature was at 30 ℃. RESULTS There was a good linear within the range of 0.019 08-0.229 0 mg·mL-1 for Gallic acid with r=0.999 6. The average recovery was 101.52% with RSD=1.40%(n=6). CONCLUDION The method is simple, accurate, reproducible and can be used for the quality control of Liquidambar taiwaniana Hance. |
| Key words: HPLC leaf of Liquidambar taiwaniana Hance gallic acid |
