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| 摘要: |
| 目的 建立老慢清糖浆中异秦皮啶的含量测定方法。方法 采用反相高效液相色谱法。色谱柱：Shim-pack VP-ODS C18柱(250 mm×4.6 mm，5 μm)，流动相：乙腈-0.2%磷酸(19∶81)，检测波长：344 nm，流速：1.0 mL·min-1，柱温：30 ℃。结果 异秦皮啶在0.214~1.922 μg内线性关系良好，相关系数r=0.999 9，平均回收率为99.1%，RSD=1.2%(n=9)。 结论 该方法简便、准确、重复性好，可用于老慢清糖浆的质量控制。 |
| 关键词: 高效液相色谱法 老慢清糖浆 异秦皮啶 |
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| Determination of Isofraxidin in Laomanqing Syrup |
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TANG Dezhi
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| Abstract: |
| OBJECTIVE To determine the quantity of isofraxidin in Laomanqing syrup．METHODS RP-HPLC was used．Chromatographic conditions: Shim-pack VP-ODS C18 column (250 mm×4.6mm, 5 μm), the mobile phase consisted of acetonitrile-0.2% phosphoric acid aqueous solution(19∶81)，the flow rate 1.0 mL·min-1，the detective wavelength 344 nm and column temperature 30 ℃. RESULTS The calibration curve was linear in the range of 0.214-1.922 μg for the isofraxidin(r=0.999 9)．The average recovery was 99.1% and the RSD was 1.2%(n=9)．CONCLUSION The method is simple and accurate with good repeatability, which may be used for the quality control of Laomanqing syrup. |
| Key words: HPLC Laomanqing syrup isofraxidin |
