| 引用本文: | 刘庆丰,李中东,刘珏,施孝金*,钟明康.HPLC-ELSD测定三黄合剂中黄芪甲苷的含量[J].中国现代应用药学,2010,27(4):355-358. |
| .Determination of Astragaloside IV in Sanhuang Mixture by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(4):355-358. |
|
|
| 摘要: |
| 目的 建立三黄合剂中黄芪甲苷含量测定的HPLC方法并制定该制剂的质量控制指标。方法 采用Diamonsil C18柱，流动相为乙腈-水(35∶65)，流速：1.0 mL·min-1；漂移管温度为95 ℃，氮气流速为3.0 L·min-1。结果 进样量在0.25~5.00 μg内，黄芪甲苷浓度的对数值lgC与峰面积积分的对数值lgA之间呈良好的线性关系，r=0.999 3。结论 本法操作简便，准确，可用于三黄合剂的质量控制。 |
| 关键词: 三黄合剂 黄芪甲苷 高效液相色谱-蒸发光散射检测器 含量测定 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Determination of Astragaloside IV in Sanhuang Mixture by HPLC-ELSD |
|
LIU Qingfeng LI Zhongdong LIU Jue SHI Xiaojin* ZHONG Mingkang
|
| Abstract: |
| OBJECTIVE To establish a method using HPLC coupled with ELSD for determination of astragaloside IV in Sanhuang mixture and formulate a standard for qulity control. METHODS A Diamonsil C18 column(200 mm×4.6 mm, 5 μm) was used, acetonitrile-water (35∶65) was used as mobile phase and the flow rate was 1.0 mL·min-1. The temperature of drift tube for ELSD was 95 ℃ and the flow rate of nitrogen was 3.0 L·min-1. RESULTS The linear range for astragaloside IV was 0.25-5.00 μg (r=0.999 3), the RSDs of intra- and inter-day were less than 2.0%. CONCLUSION The method is simple, accurate and suitable for quality control of Sanhuang mixture. |
| Key words: Sanhuang mixture astragaloside IV HPLC-ELSD determination |
