| 引用本文: | 陈志明,虞燕华,吴二明,李卫一,汪洋.HPLC测定注射用亚锡依替菲宁的含量[J].中国现代应用药学,2010,27(3):258-260. |
| .Determination of Etifenin and Stannous Chloride for Injection by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(3):258-260. |
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| 摘要: |
| 目的 建立用HPLC测定注射用亚锡依替菲宁(EHIDA药盒)含量的方法。方法 采用Waters C18(4.6 mm×150 mm，5 μm)的色谱柱，以甲醇-水-磷酸(55∶45∶0.075)为流动相，检测波长为220 nm，流速1.0 mL·min-1。结果 依替菲宁在32~384 μg·mL-1内成线性关系，Y=1 789 041X+91 432(r=0.999 92)平均回收率为98.77%，RSD为0.43%。结论 本方法操作简便，结果准确，可用于EHIDA药盒含量的快速定量分析。 |
| 关键词: 高效液相色谱法 依替菲宁 含量测定 |
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| Determination of Etifenin and Stannous Chloride for Injection by HPLC |
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CHEN Zhiming YU Yanhua WU Erming LI Weiyi WANG Yang
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| Abstract: |
| OBJECTIVE To establish a method for determination of etifenin and stannous chloride for injection(EHIDA kit). METHODS HPLC was adopted with Waters C18(4.6 mm×150 mm, 5 μm) column, methanol-water-phosphoric acid (55∶45∶0.075) was used as the mobile phase. The flow rate and detection wavelength was 1.0 mL·min-1, 220 nm, respectively. RESULTS The linear rage of etifenin were 32-384 μg·mL-1, r=0.999 92, the average recovery was 98.77%, RSD was 0.43%(n=7). CONCLUSION The method is simple, accurate and suitable for the determination of EHIDA kit. |
| Key words: HPLC etifenin content determination |
