| 引用本文: | 程竹,李凌云,范红梅,刘志海,胡秋燕.柴白安神合剂的质量控制研究[J].中国现代应用药学,2010,27(3):251-255. |
| .Study on Quality Control of Chaibaiansheng Mixture[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(3):251-255. |
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| 摘要: |
| 目的 建立柴白安神合剂的质量标准，有效控制柴白安神合剂的质量。方法 采用薄层色谱法(TLC)对柴胡、百合、白芍、五味子、川芎进行定性鉴别，高效液相色谱法(HPLC)对黄芩苷进行含量测定。结果 TLC可定性鉴别柴胡、百合、白芍、五味子、川芎5味药材，且Rf值合理，重复性好；HPLC中黄芩苷在0.103 2~1.032 μg内线性关系良好(r=0.999 8)，平均回收率为99.5%，RSD为1.4%(n=6)。结论 该质量控制方法准确，简单、专属性强，可有效控制柴白安神合剂的质量。 |
| 关键词: 柴白安神合剂 质量标准 薄层色谱法 高效液相色谱法 黄芩苷 |
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| Study on Quality Control of Chaibaiansheng Mixture |
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CHENG Zhu1 LI Lingyun2 FAN Hongmei2 LIU Zhihai2 HU Qiuyan3
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| Abstract: |
| OBJECTIVE To establish the quality standards for quality control of Chaibaiansheng mixture. METHODS TLC was adopted to identify Radix Bupleurum, Bulbus Lily, Radix Paeoniae Alba, Fructus Schisandrae Chinensis and Rhizoma Chuanxiong, and a HPLC method was used to test the content of Baicalin. RESULTS TLC can identify these extraction: Radix Bupleuri, Bulbus Lilii, Radix Paeoniae Alba, Fructus Schisandrae Chinensis and Rhizoma Chuanxiong, and the Rf is reasonable and repeatable; Baicalin has good linearity relationship by HPLC in 0.103 2-1.032 μg(r=0.999 8), the average recovery is 99.7%, RSD is 1.5%(n=6). CONCLUSION This method of qualitative control is precise, easy and exclusive, and has good effect on quality control of Chaibaiansheng mixture. |
| Key words: Chaibaiansheng mixture quality control TLC HPLC Baicalin |
