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摘要: |
目的建立高效液相色谱法测定复方双花藤止痒搽剂中苦参碱的含量。方法采用色谱柱为Thermo ODS Hypersil(4.6 mm×250 mm,5 μm),以乙睛-磷酸盐缓冲液(取磷酸二氢钾1.36 g,加1 mL三乙胺,加水至1 000 mL,用磷酸调pH至5.5)(4∶96)为流动相,检测波长220 nm,流速1.0 mL·min-1,柱温为35 ℃。结果苦参碱在0.43~21.44 μg内有良好的线性关系(r=0.999 9),平均回收率为100.40%,RSD为1.93%(n=6)。结论该方法结果准确可靠,重复性好,可用于复方双花藤止痒搽剂中苦参碱的含量测定。 |
关键词: 反相高效液相色谱法 复方双花藤止痒搽剂 苦参碱 |
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Determination the Content of Matrine in Fufang Shuanghuateng Zhiyang Liniment by RP-HPLC |
QIU Jin,LI Qiang,PANG Yunjuan
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Abstract: |
OBJECTIVE To establish a RP-HPLC method for the determination of matrine in Fufang Shuanghuateng Zhiyang liniment.METHODS The Thermo ODS Hypersil column(250 mm×4.6 mm,5 μm)was used with the acetonitrile-phosphate buffer solution(contained 1.36 g potassium dihydrogen phosphate and 1 mL triethylamine,add water to 1 000 mL adjusted pH to 5.5 with phosphate acid)(4∶96)as mobile phase,the detection wavelength was 220 nm,the flow rate was 1.0 mL·min-1,the temperature of the column was 35 ℃。RESULTS The linear range of matrine was at 0.43-21.44 μg(r=0.999 9,the average recovery was 100.40% with RSD of 1.93%(n=6).CONCLUSION The method is accurate and reliable,it can be used for the determination of matrine in Fufang Shuanghuateng Zhiyang liniment. |
Key words: RP-HPLC Fufang Shuanghuateng Zhiyang liniment matrine |