| 引用本文: | 唐靓，李跃中，陆明旸，王晗，李昉赟，王茵*.微生物法与HPLC测定药品及食品中叶酸含量的比较及应用[J].中国现代应用药学,2009,(12):1016-1019. |
| TANG Liang, LI Yuezhong, LU Mingyang, WANG Hang, LI Fangyun,WANG Yin*.Comparison and Application of the Determination Method of Folic Acid in Medicament and Foods by Bioassay and HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(12):1016-1019. |
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| 摘要: |
| 目的 建立微生物法测定药品与食品中叶酸含量的方法，解决液相色谱不能测定食品化合物形式叶酸含量的不足，为孕妇叶酸日摄入量的统计提供理论依据。 方法 利用酪乳酸杆菌对叶酸生长的依赖性，测定其菌液光密度值，得到叶酸含量。 结果 检测3个种类的样品，得到了批内、批间、回收率的数据，并与液相色谱的检测结果做了比较。 结论 微生物法稳定可靠，比液相色谱法应用更广。 |
| 关键词: 微生物法 液相色谱 叶酸 |
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| Comparison and Application of the Determination Method of Folic Acid in Medicament and Foods by Bioassay and HPLC |
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TANG Liang, LI Yuezhong, LU Mingyang, WANG Hang, LI Fangyun,WANG Yin*
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| Abstract: |
| OBJECTIVE To explore a method to determine the folic acid content in medicament and foods by bioassay, to resolve the problem that folic acid content of foods can’t be determined by HPLC, and to provide a way to calculate the ingestion of folic acid per day for pregnant woman. METHODS Utilizing the growth of Lactobacillus Casei (L.C) depending on folic acid to determine bacterial suspension’s absorbency, the content of folic acid is obtained. RESULTS Through examining three kinds of samples, the coefficient variations of within-run, between-run and the rate of recovery have been got, and the result of the HPLC’s was compared. CONCLUSION Bioassay is more stable and applicable than HPLC. |
| Key words: bioassay HPLC folic acid |
