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| 摘要: |
| 目的 建立高效液相色谱法测定延胡索酸泰妙菌素含量。方法 采用Agilent C18 (150 mm×4.6 mm , 5 μm), 流动相为0.1 mol·mL-1碳酸铵缓冲液(取碳酸铵10 g，加水800 mL使溶解，加24 mL 6%的高氯酸水溶液，加水至1 000 mL)-乙腈-甲醇(27∶23∶50)；检测波长为212 nm。结果 线性范围为0.25～0.75 mg·mL-1 (r=0.999 4)；平均回收率为99.3% (RSD=1.2%)。结论 方法快捷，准确，重复性好。 |
| 关键词: 延胡索酸泰妙菌素 高效液相色谱法 含量 |
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| Determination the Content of Tiamulin Fumarate by RP-HPLC |
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CHEN Aiying1, GUAN Chen2，XU Shifang1，JIANG Lixia1*
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| Abstract: |
| OBJECTIVE To establish a method for determination the content of tiamulin fumarate. METHODS RP-HPLC was adopted, the column was Agilent C18 (150 mm×4.6 mm, 5 μm), the mobile phase was 0.1 mol·mL-1 ammonium carbonate buffer (add 10 g ammonium carbonate to a 1 000 mL volumetric flask and dissolve in about 800 mL of water. Add 24 mL 6% of perchloric acid and dilute with water to volume) -acetonitril-methanol (27∶23∶50). UV dector wavelength was 212 nm. RESULTS The calibration is linear between 0.25-0.75 mg·mL-1. The mean recovery was 99.3%, RSD%=1.2%. CONCLUSION This method inspecting tiamulin fumarate is rapid, accurate and reproducible. |
| Key words: tiamulin fumarate HPLC content |
