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| 摘要: |
| 目的以指纹图谱方式，研究黄连和厚朴乙醇合提和分提提取物中化学成分的差异。方法采用常规制备方法得黄连和厚朴醇提合提和分提的提取物，采用HPLC考察并确定最佳的检测波长，最佳色谱柱等色谱条件，并采用最佳色谱条件对黄连厚朴配伍不同制备方法的提取物进行测定，比较不同提取物的指纹图谱的差异。结果不同提取物中化学成分组成存在差异，且提取物中主要化学成分的提取率也存在差异。结论黄连厚朴醇提合提和醇提分提提取物中主要化学成分组成存在差异。 |
| 关键词: 黄连 厚朴 配伍 指纹图谱 |
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| Fingerprint Comparison of the Extraction of Rhizoma Coptidis and Cortex Magnoliae Officinalis Extracted by Ethanol Together and Single |
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MENG Bin, ZHANG Zhenqiu
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| Abstract: |
| OBJECTIVE To study on the differences of chemical composition between extraction of Rhizoma Coptidis and Cortex Magnoliae extracted by ethanol together and single，with fringerprint technology. METHODS The extracts of Rhizoma Coptidis and Cortex Magnoliae extracted by ethanol together and single under the same process. The comparison was done under the different wavelengths and different brand columns, HPLC method determined the extract's fingerprint within the range of optimized conditions. RESULTS The ratio of compositions and its main chemical substances are different between the two extracts, and the extract rate of the main chemical substances are different, too. CONCLUSION There are some chemical substances and composition's ratio differences in the extracts of Rhizoma Coptidis and Cortex Magnoliae Officinalis by ethanol together and single. |
| Key words: rhizoma coptidis cortex magnoliae officinalis combination fingerprint |
