| 引用本文: | 杨云1，肖功胜1，闻永举2，田润涛3.黄芩及其炮制品黄芩片的HPLC指纹图谱研究[J].中国现代应用药学,2009,(9):720-724. |
| YANG Yun1, XIAO Gongsheng1, WEN Yongju2, TIAN Runtao3.Study on the HPLC Fingerprint of Radix Scutellariae and Its Processed Pieces[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(9):720-724. |
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| 摘要: |
| 目的对黄芩药材及其炮制品黄芩片进行HPLC指纹图谱研究。方法选择HPLC-UV指纹图谱。色谱条件：Hypersil C18柱（200 mm×5.0 mm，5 μm）；流动相为甲醇-0.4%磷酸水溶液-乙腈线性梯度洗脱；流速：1.0 mL·min-1；柱温28 ℃；检测波长277 nm。结果10批不同产地正品黄芩进行规范炮制加工后得到的黄芩片指纹图谱无明显差异，14个不同产地黄芩药材的指纹图谱也具有极高的相似度。结论该方法重复性好，可为黄芩药材及其饮片的质量控制提供科学依据。 |
| 关键词: 黄芩 HPLC指纹图谱 黄芩饮片 |
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| Study on the HPLC Fingerprint of Radix Scutellariae and Its Processed Pieces |
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YANG Yun1, XIAO Gongsheng1, WEN Yongju2, TIAN Runtao3
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| Abstract: |
| OBJECTIVE To study the HPLC fingerprints of Radix Scutellariae and its processed pieces. METHODS Selecting HPLC-UV fingerprints. Using a Hypersil C18 column (200 mm×5.0 mm, 5 μm). The mobile phase was linear gradient elute with methanol-0.4% phosphoric acid-acetonitrile. Flow rate was 1.0 mL·min-1. Column temperature was 28 ℃. Detection wavelength was set at 277 nm. RESULTS There was no evident difference among the fingerprints of 10 batches of Radix Scutellariae that were normatively manufactured and processed,and the similarity of 14 batches of Radix Scutellariae was very well. CONCLUSION This method recurs well, and can be used to provide scientific basis for Radix Scutellariae and its processed pieces quanlity control mode. |
| Key words: Radix Scutellariae HPLC-fingerprint processed Radix Scutellariae pieces |
