| 引用本文: | 匡荣1,2，孙意国1，郑筱祥1*，倪维芳2，朱社敏2.硫辛酸通过抑制NF-κB-iNOS-NO信号保护氧化应激诱导的PC12细胞损伤[J].中国现代应用药学,2009,(7):538-541. |
| KUANG Rong1,2, SUN Yiguo1, ZHENG Xiaoxiang1*, NI Weifang2, ZHU Shemin2.Lipoic Acid Protected PC12 Cells from Oxidative Stress-induced Damage by inhibiting the NF-κB- iNOS-NO Signal Pathway[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(7):538-541. |
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| 摘要: |
| 目的 研究硫辛酸对氧化应激的PC12细胞NF-κB - iNOS-NO信号通路的影响。方法 用终浓度为0.4 mmol·L-1的H2O2损伤PC12细胞，用MTT法测定细胞存活率；用激光共聚焦法(LSCM)检测细胞内一氧化氮的动态变化；用RT-PCR法检测iNOS mRNA和NF-κB p65 mRNA表达量的变化。结果 0.4 mmol·L-1的H2O2处理PC12细胞4 h，细胞存活率为27.9%(P<0.01)，硫辛酸10 mg·L-1可以提高H2O2损伤的PC12细胞存活率(65.4%，P<0.01)；LSCM检测显示，DAF-2荧光强度的比值(Ft/F0)的最大值由损伤模型组的5.34下降到1.80；和模型组比较，iNOS mRNA和NF-κB p65 mRNA的表达明显下调 (P<0.05或P<0.01)。结论 硫辛酸可能通过抑制NF-κB - iNOS-NO信号减轻氧化应激诱导的PC12细胞损伤。 |
| 关键词: 硫辛酸 过氧化氢 PC12细胞 一氧化氮 iNOS mRNA NF-κB p65 mRNA |
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| Lipoic Acid Protected PC12 Cells from Oxidative Stress-induced Damage by inhibiting the NF-κB- iNOS-NO Signal Pathway |
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KUANG Rong1,2, SUN Yiguo1, ZHENG Xiaoxiang1*, NI Weifang2, ZHU Shemin2
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| Abstract: |
| OBJECTIVE To investigate the effect of lipoic acid(LA) on NF-κB - iNOS-NO signal pathway in oxidative stress-induced PC12 cells. METHODS After PC12 cells were treated with 0.4 mmol·L-1 H2O2 and different concentrations of LA, the cell viability was measured by MTT, the concentration of nitric oxide in intracellular cells was evaluated using laser-scanning confocal microscopy(LSCM) method and the expressions of NF-κB p65, iNOS mRNA were detected by RT-PCR. RESULTS Cell viability were significantly elevated by LA from 27.9% to 65.4%. Compared with H2O2-treated cells, the concentration of nitric oxide was lowed by LA through judging the change of DAF-2 Ft/F0 from 5.34 to 1.80. Meanwhile, the levels of NF-κB p65 and iNOS mRNA were decreased (P<0.05 or P<0.01). CONCLUSION Lipoic acid could protect PC12 cells from oxidative stress-induced damage by inhibiting the NF-κB - iNOS-NO signal pathway. |
| Key words: lipoic acid H2O2 PC12 cells NO iNOS mRNA NF-κB mRNA |
